biostudies-literatureGuo JEarmarked Fund for China Agriculture Research System1089389https://www.ebi.ac.uk/biostudies/studies/S-EPMC9845247biostudies-literatureUnknown13As an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F₂ segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content variety Jihuatian 1 and the low sucrose content line PI478819, were used as materials to map a quantitative trait locus (QTL) associated with sucrose content in peanut kernels. Four QTLs were initially located on chromosomes A03 and A06 based on BSA-seq technology, and multiple kompetitive allele-specific PCR markers were developed based on single-nucleotide polymorphisms (SNPs) in the intervals. The markers were genotyped in the RIL population and finely mapped to a stable QTL, <i>qSUCA0Frontiers in geneticsMapping of a QTL associated with sucrose content in peanut kernels using BSA-seq.PMC9845247CARS-13Liu HLi HCui MDong WTian MQin LLi CZhang MMiao LHan SSun ZXu JLuo DLi XGuo JZhao QZhang XQi FHuang BfalseMapping of a QTL associated with sucrose content in peanut kernels using BSA-seq.As an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F₂ segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content variety Jihuatian 1 and the low sucrose content line PI478819, were used as materials to map a quantitative trait locus (QTL) associated with sucrose content in peanut kernels. Four QTLs were initially located on chromosomes A03 and A06 based on BSA-seq technology, and multiple kompetitive allele-specific PCR markers were developed based on single-nucleotide polymorphisms (SNPs) in the intervals. The markers were genotyped in the RIL population and finely mapped to a stable QTL, <i>qSUCA02022-01-01T00:00:00Z20222025-04-04T13:53:03.569Z2025-04-04T13:53:03.569ZS-EPMC98452473668590910.3389/fgene.2022.1089389