{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tham CY"],"funding":["MOH | National Medical Research Council"],"pagination":["281"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9845338"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Telomeres are specialized nucleoprotein structures at the ends of linear chromosomes. The progressive shortening of steady-state telomere length in normal human somatic cells is a promising biomarker for age-associated diseases. However, there remain substantial challenges in quantifying telomere length due to the lack of high-throughput method with nucleotide resolution for individual telomere. Here, we describe a workflow to capture telomeres using newly designed telobaits in human culture cell lines as well as clinical patient samples and measure their length accurately at nucleotide resolution using single-molecule real-time (SMRT) sequencing. Our results also reveal the extreme heterogeneity of telomeric variant sequences (TVSs) that are dispersed throughout the telomere repeat region"],"journal":["Nature communications"],"pubmed_title":["High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform."],"pmcid":["PMC9845338"],"funding_grant_id":["NMRC/CIRG/1481/2017","MOH-000153"],"pubmed_authors":["Xu L","Liu J","Koh JYP","Hwang WYK","Yan T","Koh AS","Zhang S","Lee GS","Teh BT","Osato M","Li S","Hong Z","Song HW","Poon L","Cai Y","Tham CY","Tan P","Ramlee MK","Teoh VSI"],"additional_accession":[]},"is_claimable":false,"name":"High-throughput telomere length measurement at nucleotide resolution using the PacBio high fidelity sequencing platform.","description":"Telomeres are specialized nucleoprotein structures at the ends of linear chromosomes. The progressive shortening of steady-state telomere length in normal human somatic cells is a promising biomarker for age-associated diseases. However, there remain substantial challenges in quantifying telomere length due to the lack of high-throughput method with nucleotide resolution for individual telomere. Here, we describe a workflow to capture telomeres using newly designed telobaits in human culture cell lines as well as clinical patient samples and measure their length accurately at nucleotide resolution using single-molecule real-time (SMRT) sequencing. Our results also reveal the extreme heterogeneity of telomeric variant sequences (TVSs) that are dispersed throughout the telomere repeat region","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-06-03T18:30:27.576Z","creation":"2025-04-19T12:37:57.216Z"},"accession":"S-EPMC9845338","cross_references":{"pubmed":["36650155"],"doi":["10.1038/s41467-023-35823-7"]}}