{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Ayechu-Muruzabal V"],"pubmed_abstract":["<h4>Introduction</h4>Early life exposure to non-digestible oligosaccharides (NDO) or microbial components is known to affect immune development. NDO in combination with a TLR9 agonist mimicking bacterial triggers (CpG) promoted the secretion of galectins through unknown pathways. We aimed to study the contribution of exosomes in epithelial galectin secretion and subsequent immunoregulation upon exposure to a mixture of NDO by inhibiting exosome biogenesis.<h4>Methods</h4>Human intestinal epithelial cells (IEC) (FHs 74 Int or HT-29) were apically exposed to 2'-fucosyllactose (2'FL) and short-chain galacto- and long-chain fructo-oligosaccharides (GF), alone or with CpG. Basolaterally, non-activated or αCD3/CD28-activated peripheral blood mononuclear cells (PBMC) were added. After 24 h incuba"],"journal":["Frontiers in immunology"],"pagination":["1026031"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9846635"],"repository":["biostudies-literature"],"pubmed_title":["Epithelial-derived galectin-9 containing exosomes contribute to the immunomodulatory effects promoted by 2'-fucosyllactose and short-chain galacto- and long-chain fructo-oligosaccharides."],"pmcid":["PMC9846635"],"pubmed_authors":["de Boer M","Kraneveld AD","Van't Land B","Berends AJ","Ayechu-Muruzabal V","Willemsen LEM","Blokhuis B","Garssen J"],"additional_accession":[]},"is_claimable":false,"name":"Epithelial-derived galectin-9 containing exosomes contribute to the immunomodulatory effects promoted by 2'-fucosyllactose and short-chain galacto- and long-chain fructo-oligosaccharides.","description":"<h4>Introduction</h4>Early life exposure to non-digestible oligosaccharides (NDO) or microbial components is known to affect immune development. NDO in combination with a TLR9 agonist mimicking bacterial triggers (CpG) promoted the secretion of galectins through unknown pathways. We aimed to study the contribution of exosomes in epithelial galectin secretion and subsequent immunoregulation upon exposure to a mixture of NDO by inhibiting exosome biogenesis.<h4>Methods</h4>Human intestinal epithelial cells (IEC) (FHs 74 Int or HT-29) were apically exposed to 2'-fucosyllactose (2'FL) and short-chain galacto- and long-chain fructo-oligosaccharides (GF), alone or with CpG. Basolaterally, non-activated or αCD3/CD28-activated peripheral blood mononuclear cells (PBMC) were added. After 24 h incuba","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-03-17T15:21:47.543Z","creation":"2025-04-05T19:50:34.181Z"},"accession":"S-EPMC9846635","cross_references":{"pubmed":["36685520"],"doi":["10.3389/fimmu.2022.1026031"]}}