biostudies-literatureUnknown12Bai Z<i>Faecalibacterium prausnitzii</i> is a beneficial human gut microbe and a candidate for next-generation probiotics. With probiotics now being used in clinical treatments, concerns about their safety and side effects need to be considered. Therefore, it is essential to obtain a comprehensive understanding of the genetic diversity, functional characteristics, and potential risks of different <i>F. prausnitzii</i> strains. In this study, we collected the genetic information of 84 <i>F . prausnitzii</i> strains to conduct a pan-genome analysis with multiple perspectives. Based on single-copy genes and the sequences of 16S rRNA and the compositions of the pan-genome, different phylogenetic analyses of <i>F. prausnitzii</i> strains were performed, which showed the genetic diversity among them.Frontiers in cellular and infection microbiology919701https://www.ebi.ac.uk/biostudies/studies/S-EPMC9846645biostudies-literatureComprehensive analysis of 84 <i>Faecalibacterium prausnitzii</i> strains uncovers their genetic diversity, functional characteristics, and potential risks.PMC9846645Sun LLi GLiu YHan MChen LFang FZhang NBai ZJin YMao YfalseComprehensive analysis of 84 <i>Faecalibacterium prausnitzii</i> strains uncovers their genetic diversity, functional characteristics, and potential risks.<i>Faecalibacterium prausnitzii</i> is a beneficial human gut microbe and a candidate for next-generation probiotics. With probiotics now being used in clinical treatments, concerns about their safety and side effects need to be considered. Therefore, it is essential to obtain a comprehensive understanding of the genetic diversity, functional characteristics, and potential risks of different <i>F. prausnitzii</i> strains. In this study, we collected the genetic information of 84 <i>F . prausnitzii</i> strains to conduct a pan-genome analysis with multiple perspectives. Based on single-copy genes and the sequences of 16S rRNA and the compositions of the pan-genome, different phylogenetic analyses of <i>F. prausnitzii</i> strains were performed, which showed the genetic diversity among them.2022-01-01T00:00:00Z20222025-04-04T13:53:22.34Z2025-04-04T13:53:22.34ZS-EPMC98466453668368610.3389/fcimb.2022.919701