biostudies-literatureUnknown1118Yanai ZThe mayfly genus <i>Alainites</i> Waltz & McCafferty, 1994 encompassed 20 species and was represented across the West Palaearctic region by six species. Based on morphological (nymphal characters) and molecular (mitochondrial COI sequences) evidence, two new species are described: <i>A.bengunn</i> <b>sp. nov.</b> from Sardinia and <i>A.gasithi</i> <b>sp. nov.</b> from Israel. Both species are confined to narrow distribution ranges, in line with most of their congeners from the region. The key nymphal traits are discussed and identified to distinguish species in the group.ZooKeys73-95https://www.ebi.ac.uk/biostudies/studies/S-EPMC9848644biostudies-literatureTwo new species of <i>Alainites</i> (Ephemeroptera, Baetidae) from the Mediterranean biodiversity hotspot.PMC9848644Yanai ZGattolliat JLSroka PfalseTwo new species of <i>Alainites</i> (Ephemeroptera, Baetidae) from the Mediterranean biodiversity hotspot.The mayfly genus <i>Alainites</i> Waltz & McCafferty, 1994 encompassed 20 species and was represented across the West Palaearctic region by six species. Based on morphological (nymphal characters) and molecular (mitochondrial COI sequences) evidence, two new species are described: <i>A.bengunn</i> <b>sp. nov.</b> from Sardinia and <i>A.gasithi</i> <b>sp. nov.</b> from Israel. Both species are confined to narrow distribution ranges, in line with most of their congeners from the region. The key nymphal traits are discussed and identified to distinguish species in the group.2022-01-01T00:00:00Z20222025-04-18T21:15:37.238Z2025-04-07T09:14:46.297ZS-EPMC98486443676180310.3897/zookeys.1118.84643