biostudies-literatureUnknown90Rosenblad MANon-coding RNA (ncRNA) genes play important, but incompletely understood, roles in various cellular processes, notably translation and gene regulation. A recent report on the detection of the ncRNA Signal Recognition Particle gene in the nuclear ribosomal internal transcribed spacer region of several species of three genera of ectomycorrhizal basidiomycetes prompted a more thorough bioinformatics search for additional ncRNA genes in the full fungal ribosomal operon. This study reports on the detection of three ncRNA genes hitherto not known from the fungal ribosomal region: nuclear RNase P RNA, RNase MRP RNA, and a possible snoRNA U14 in a total of five species of <i>Auricularia</i> and <i>Inocybe</i>. We verified their presence through resequencing of independent specimens. Two completed <i>Auricularia</i> genomes were found to lack these ncRNAs elsewhere than in the ribosomal operon, suggesting that these are functional genes. It seems clear that ncRNA genes play a larger role in fungal ribosomal genetics than hitherto thought.MycoKeys203-213https://www.ebi.ac.uk/biostudies/studies/S-EPMC9849065biostudies-literatureEvidence for further non-coding RNA genes in the fungal rDNA region.PMC9849065Wurzbacher CWalker ARosenblad MALarsson EThongklang NNilsson RHfalseEvidence for further non-coding RNA genes in the fungal rDNA region.Non-coding RNA (ncRNA) genes play important, but incompletely understood, roles in various cellular processes, notably translation and gene regulation. A recent report on the detection of the ncRNA Signal Recognition Particle gene in the nuclear ribosomal internal transcribed spacer region of several species of three genera of ectomycorrhizal basidiomycetes prompted a more thorough bioinformatics search for additional ncRNA genes in the full fungal ribosomal operon. This study reports on the detection of three ncRNA genes hitherto not known from the fungal ribosomal region: nuclear RNase P RNA, RNase MRP RNA, and a possible snoRNA U14 in a total of five species of <i>Auricularia</i> and <i>Inocybe</i>. We verified their presence through resequencing of independent specimens. Two completed <i>Auricularia</i> genomes were found to lack these ncRNAs elsewhere than in the ribosomal operon, suggesting that these are functional genes. It seems clear that ncRNA genes play a larger role in fungal ribosomal genetics than hitherto thought.2022-01-01T00:00:00Z20222026-06-24T03:12:35.349Z2025-04-04T11:50:12.202ZS-EPMC98490653676042510.3897/mycokeys.90.84866