biostudies-literatureUnknown91Zhang ZThe fungal genus <i>Phyllosticta</i> has been reported from all around the world and accommodates numerous pathogenic and endophytic species isolated from a wide range of plant hosts. Based on multilocus phylogenies from a combined dataset of genes encoding internal transcribed spacer (ITS), large subunit of ribosomal RNA (LSU rDNA), translation elongation factor 1 alpha (TEF1α), actin (ACT) and glycerol-3-phosphate dehydrogenase (GPDH), in conjunction with morphological characteristics, we describe two new species <i>P.oblongifoliae</i> <b>sp. nov.</b> and <i>P.pterospermi</i> <b>sp. nov.</b>, as well as a new Chinese record <i>P.capitalensis</i>. Their similarity and dissimilarity to morphologically-allied and phylogenetically-related species are also annotated and discussed.MycoKeys1-23https://www.ebi.ac.uk/biostudies/studies/S-EPMC9849093biostudies-literatureMorphological and phylogenetic analyses reveal two new species and a new record of <i>Phyllosticta</i> (Botryosphaeriales, Phyllostictaceae) from Hainan, China.PMC9849093Liu XZhang ZZhang XMeng ZfalseMorphological and phylogenetic analyses reveal two new species and a new record of <i>Phyllosticta</i> (Botryosphaeriales, Phyllostictaceae) from Hainan, China.The fungal genus <i>Phyllosticta</i> has been reported from all around the world and accommodates numerous pathogenic and endophytic species isolated from a wide range of plant hosts. Based on multilocus phylogenies from a combined dataset of genes encoding internal transcribed spacer (ITS), large subunit of ribosomal RNA (LSU rDNA), translation elongation factor 1 alpha (TEF1α), actin (ACT) and glycerol-3-phosphate dehydrogenase (GPDH), in conjunction with morphological characteristics, we describe two new species <i>P.oblongifoliae</i> <b>sp. nov.</b> and <i>P.pterospermi</i> <b>sp. nov.</b>, as well as a new Chinese record <i>P.capitalensis</i>. Their similarity and dissimilarity to morphologically-allied and phylogenetically-related species are also annotated and discussed.2022-01-01T00:00:00Z20222026-04-08T12:44:37.756Z2025-04-05T19:42:07.321ZS-EPMC98490933676088610.3897/mycokeys.91.84803