biostudies-literatureToledano SIsrael Science Foundation41https://www.ebi.ac.uk/biostudies/studies/S-EPMC9852426biostudies-literatureUnknown14(1)The semaphorin guidance factors receptor plexin-A2 transduces sema6A and sema6B signals and may mediate, along with plexin-A4, the anti-angiogenic effects of sema6A. When associated with neuropilins plexin-A2 also transduces the anti-angiogenic signals of sema3B. Here we show that inhibition of plexin-A2 expression in glioblastoma derived cells that express wild type p53 such as U87MG and A172 cells, or in primary human endothelial cells, strongly inhibits cell proliferation. Inhibition of plexin-A2 expression in U87MG cells also results in strong inhibition of their tumor forming ability. Knock-out of the plexin-A2 gene in U87MG cells using CRISPR/Cas9 inhibits cell proliferation which is rescued following plexin-A2 re-expression, or expression of a truncated plexin-A2 lacking its extraceCell death & diseasePlexin-A2 enables the proliferation and the development of tumors from glioblastoma derived cells.PMC9852426188/16Kessler ONeufeld GSabag ADLiburkin-Dan TToledano SIlan NfalsePlexin-A2 enables the proliferation and the development of tumors from glioblastoma derived cells.The semaphorin guidance factors receptor plexin-A2 transduces sema6A and sema6B signals and may mediate, along with plexin-A4, the anti-angiogenic effects of sema6A. When associated with neuropilins plexin-A2 also transduces the anti-angiogenic signals of sema3B. Here we show that inhibition of plexin-A2 expression in glioblastoma derived cells that express wild type p53 such as U87MG and A172 cells, or in primary human endothelial cells, strongly inhibits cell proliferation. Inhibition of plexin-A2 expression in U87MG cells also results in strong inhibition of their tumor forming ability. Knock-out of the plexin-A2 gene in U87MG cells using CRISPR/Cas9 inhibits cell proliferation which is rescued following plexin-A2 re-expression, or expression of a truncated plexin-A2 lacking its extrace2023-01-01T00:00:00Z2023 Jan2026-05-28T23:02:19.515Z2024-10-16T17:14:28.294ZS-EPMC98524263665811410.1038/s41419-023-05554-0