biostudies-literatureHong HSNIDCR NIH HHSNIDDK NIH HHSNIAID NIH HHSNCI NIH HHSeabm8182https://www.ebi.ac.uk/biostudies/studies/S-EPMC9853437biostudies-literatureUnknown7(77)T cell proliferation and cytokine production are bioenergetically and biosynthetically costly. The inability to meet these metabolic demands results in altered differentiation, accompanied by impaired effector function, and attrition of the immune response. Interleukin-17-producing CD4 T cells (T_H17s) are mediators of host defense, autoimmunity, and antitumor immunity in the setting of adoptive T cell therapy. T_H17s are long-lived cells that require mitochondrial oxidative phosphorylation (OXPHOS) for effector function in vivo. Considering that T_H17s polarized under standardized culture conditions are predominately glycolytic, little is known about how OXPHOS regulates T_H17 processes, such as their ability to persist and thus contribute to protracted immune responses. Here, we modified standardized culture medium and identified a culture system that reliably induces OXPHOS dependence in T_H17s. We found that T_H17s cultured under OXPHOS conditions metabolically resembled their in vivo counterparts, whereas glycolytic cultures were dissimilar. OXPHOS T_H17s exhibited increased mitochondrial fitness, glutamine anaplerosis, and an antiapoptotic phenotype marked by high BCL-XL and low BIM. Limited mitophagy, mediated by mitochondrial fusion regulator OPA-1, was critical to apoptotic resistance in OXPHOS T_H17s. By contrast, glycolytic T_H17s exhibited more mitophagy and an imbalance in BCL-XL to BIM, thereby priming them for apoptosis. In addition, through adoptive transfer experiments, we demonstrated that OXPHOS protected T_H17s from apoptosis while enhancing their persistence in the periphery and tumor microenvironment in a murine model of melanoma. Together, our work demonstrates how metabolism regulates T_H17 cell fate and highlights the potential for therapies that target OXPHOS in T_H17-driven diseases.Science immunologyOXPHOS promotes apoptotic resistance and cellular persistence in T_H17 cells in the periphery and tumor microenvironment.PMC9853437R01 DE026728P30 CA046592R01 CA244827U24 DK097153T32 DK094775T32 CA140044T32 AI007413R37 CA237421Magnuson BShan MFranchi LOpipari AWZhang LKamada NAndren ASajjakulnukit PCarty SAZou WKryczek IHong HSXie YChen JLoesel KLei YLCorrea LOArguello RJLi ZGoldstein DRMbah NEHayashi ALin JLin LLyssiotis CAfalseOXPHOS promotes apoptotic resistance and cellular persistence in T_H17 cells in the periphery and tumor microenvironment.T cell proliferation and cytokine production are bioenergetically and biosynthetically costly. The inability to meet these metabolic demands results in altered differentiation, accompanied by impaired effector function, and attrition of the immune response. Interleukin-17-producing CD4 T cells (T_H17s) are mediators of host defense, autoimmunity, and antitumor immunity in the setting of adoptive T cell therapy. T_H17s are long-lived cells that require mitochondrial oxidative phosphorylation (OXPHOS) for effector function in vivo. Considering that T_H17s polarized under standardized culture conditions are predominately glycolytic, little is known about how OXPHOS regulates T_H17 processes, such as their ability to persist and thus contribute to protracted immune responses. Here, we modified standardized culture medium and identified a culture system that reliably induces OXPHOS dependence in T_H17s. We found that T_H17s cultured under OXPHOS conditions metabolically resembled their in vivo counterparts, whereas glycolytic cultures were dissimilar. OXPHOS T_H17s exhibited increased mitochondrial fitness, glutamine anaplerosis, and an antiapoptotic phenotype marked by high BCL-XL and low BIM. Limited mitophagy, mediated by mitochondrial fusion regulator OPA-1, was critical to apoptotic resistance in OXPHOS T_H17s. By contrast, glycolytic T_H17s exhibited more mitophagy and an imbalance in BCL-XL to BIM, thereby priming them for apoptosis. In addition, through adoptive transfer experiments, we demonstrated that OXPHOS protected T_H17s from apoptosis while enhancing their persistence in the periphery and tumor microenvironment in a murine model of melanoma. Together, our work demonstrates how metabolism regulates T_H17 cell fate and highlights the potential for therapies that target OXPHOS in T_H17-driven diseases.2022-01-01T00:00:00Z2022 Nov2025-04-21T15:11:03.658Z2025-04-21T15:11:03.658ZS-EPMC98534373639953910.1126/sciimmunol.abm8182