biostudies-literatureNora LCFAPESPCNPq1069443https://www.ebi.ac.uk/biostudies/studies/S-EPMC9853887biostudies-literatureUnknown13The demand for robust microbial cell factories that produce valuable biomaterials while resisting stresses imposed by current bioprocesses is rapidly growing. <i>Rhodosporidium toruloides</i> is an emerging host that presents desirable features for bioproduction, since it can grow in a wide range of substrates and tolerate a variety of toxic compounds. To explore <i>R. toruloides</i> suitability for application as a cell factory in biorefineries, we sought to understand the transcriptional responses of this yeast when growing under experimental settings that simulated those used in biofuels-related industries. Thus, we performed RNA sequencing of the oleaginous, carotenogenic yeast in different contexts. The first ones were stress-related: two conditions of high temperature (37 and 42°C) aFrontiers in microbiologyMining novel <i>cis</i>-regulatory elements from the emergent host <i>Rhodosporidium toruloides</i> using transcriptomic data.PMC98538872020/02207-52019/15675-02019/05026-42021/01748-52017/18922-2140212/2019-12019/04942-7Nora LCSantana IPCassiano MHASilva-Rocha Rda Silva RRGuazzaroni MEfalseMining novel <i>cis</i>-regulatory elements from the emergent host <i>Rhodosporidium toruloides</i> using transcriptomic data.The demand for robust microbial cell factories that produce valuable biomaterials while resisting stresses imposed by current bioprocesses is rapidly growing. <i>Rhodosporidium toruloides</i> is an emerging host that presents desirable features for bioproduction, since it can grow in a wide range of substrates and tolerate a variety of toxic compounds. To explore <i>R. toruloides</i> suitability for application as a cell factory in biorefineries, we sought to understand the transcriptional responses of this yeast when growing under experimental settings that simulated those used in biofuels-related industries. Thus, we performed RNA sequencing of the oleaginous, carotenogenic yeast in different contexts. The first ones were stress-related: two conditions of high temperature (37 and 42°C) a2022-01-01T00:00:00Z20222026-05-28T18:40:44.87Z2025-02-19T04:45:46.319ZS-EPMC98538873668761210.3389/fmicb.2022.1069443