{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(1)"],"submitter":["Azegami N"],"pubmed_abstract":["The contribution of disordered regions to protein function and structure is a relatively new field of study and of particular significance as their function has been implicated in some human diseases. Our objective was to analyze various deletion mutants of the bromodomain-containing protein 4 (BRD4) using native mass spectrometry to characterize the gas-phase behavior of the disordered region connected to the folded domain. A protein with a single bromodomain but no long disordered linker displayed a narrow charge distribution at low charge states, suggesting a compact structure. In contrast, proteins containing one or two bromodomains connected to a long disordered region exhibited multimodal charge distributions, suggesting the presence of compact and elongated conformers. In the presen"],"journal":["Mass spectrometry (Tokyo, Japan)"],"pagination":["A0110"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9853951"],"repository":["biostudies-literature"],"pubmed_title":["Native Mass Spectrometry of BRD4 Bromodomains Linked to a Long Disordered Region."],"pmcid":["PMC9853951"],"pubmed_authors":["Azegami N","Akashi S","Suzuki N","Konuma T","Taguchi R","Sakata Y"],"additional_accession":[]},"is_claimable":false,"name":"Native Mass Spectrometry of BRD4 Bromodomains Linked to a Long Disordered Region.","description":"The contribution of disordered regions to protein function and structure is a relatively new field of study and of particular significance as their function has been implicated in some human diseases. Our objective was to analyze various deletion mutants of the bromodomain-containing protein 4 (BRD4) using native mass spectrometry to characterize the gas-phase behavior of the disordered region connected to the folded domain. A protein with a single bromodomain but no long disordered linker displayed a narrow charge distribution at low charge states, suggesting a compact structure. In contrast, proteins containing one or two bromodomains connected to a long disordered region exhibited multimodal charge distributions, suggesting the presence of compact and elongated conformers. In the presen","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-07-15T22:26:26.052Z","creation":"2025-04-06T18:03:16.989Z"},"accession":"S-EPMC9853951","cross_references":{"pubmed":["36713808"],"doi":["10.5702/massspectrometry.A0110"]}}