biostudies-literatureLi ANational Natural Science Foundation of ChinaNational Natural Sciences Foundation of ChinaFundamental Research Funds for the Central Universities, Southwest Minzu UniversitySichuan Science and Technology Program281https://www.ebi.ac.uk/biostudies/studies/S-EPMC9854987biostudies-literatureUnknown13(2)Intramuscular fat (IMF) deposits help improve meat quality such as marbling, juicy, flavor and tenderness. Long-chain acyl-CoA dehydrogenase (<i>ACADL</i>) is a key enzyme for catalyzing fatty acid oxidation, and studies have shown <i>ACADL</i> is involved in the deposition and differentiation of intramuscular adipocytes. However, the effect of <i>ACADL</i> on intramuscular adipocytes differentiation in goats needs further study. In this study, to explore the mechanism of <i>ACADL</i> on the development of goat intramuscular adipocytes, we constructed an over-expression plasmids and a SI-RNA of <i>ACADL</i> to explore the function of <i>ACADL</i> on the development of goat IMF. It was found that overexpression of <i>ACADL</i> promoted the differentiation of goat intramuscular adipocytes, and promoted the expression of fat cell differentiation marker genes lipoprotein lipase <i>(LPL</i>), peroxisome proliferator activated receptor gamma (<i>PPARγ</i>), APETALA-2-like transcription factor gene (<i>AP2</i>), CCAT enhancer binding protein (<i>CEBPα</i>), preadipocyte Factor 1 (<i>Pref-1</i>) and CCAT enhancer binding protein (<i>CEBPβ</i>), and the opposite trend occurred after interference. In addition, we screened of this related tumor necrosis factor (TNF) signaling pathway by RNA-Seq. So, we validate the signaling pathway with inhibitor of TNF signaling pathway. In summary, these results indicate that <i>ACADL</i> promotes intramuscular adipocytes differentiation through activation TNF signaling pathway. This study provides an important basis for the mechanism of IMF development.Animals : an open access journal from MDPI<i>ACADL</i> Promotes the Differentiation of Goat Intramuscular Adipocytes.PMC98549872022JDTD003020210573207272331902154Xiong YLi XLi YZhu JQubi WLi ALin YWang YLiu Wfalse<i>ACADL</i> Promotes the Differentiation of Goat Intramuscular Adipocytes.Intramuscular fat (IMF) deposits help improve meat quality such as marbling, juicy, flavor and tenderness. Long-chain acyl-CoA dehydrogenase (<i>ACADL</i>) is a key enzyme for catalyzing fatty acid oxidation, and studies have shown <i>ACADL</i> is involved in the deposition and differentiation of intramuscular adipocytes. However, the effect of <i>ACADL</i> on intramuscular adipocytes differentiation in goats needs further study. In this study, to explore the mechanism of <i>ACADL</i> on the development of goat intramuscular adipocytes, we constructed an over-expression plasmids and a SI-RNA of <i>ACADL</i> to explore the function of <i>ACADL</i> on the development of goat IMF. It was found that overexpression of <i>ACADL</i> promoted the differentiation of goat intramuscular adipocytes, and promoted the expression of fat cell differentiation marker genes lipoprotein lipase <i>(LPL</i>), peroxisome proliferator activated receptor gamma (<i>PPARγ</i>), APETALA-2-like transcription factor gene (<i>AP2</i>), CCAT enhancer binding protein (<i>CEBPα</i>), preadipocyte Factor 1 (<i>Pref-1</i>) and CCAT enhancer binding protein (<i>CEBPβ</i>), and the opposite trend occurred after interference. In addition, we screened of this related tumor necrosis factor (TNF) signaling pathway by RNA-Seq. So, we validate the signaling pathway with inhibitor of TNF signaling pathway. In summary, these results indicate that <i>ACADL</i> promotes intramuscular adipocytes differentiation through activation TNF signaling pathway. This study provides an important basis for the mechanism of IMF development.2023-01-01T00:00:00Z2023 Jan2025-04-05T11:28:01.482Z2025-04-05T11:28:01.482ZS-EPMC98549873667082110.3390/ani13020281