biostudies-literatureXu Lthe Central Public Interest Scientific Institution Basal Research Fund for the Chinese Academy of Agricultural SciencesNational Academy of SciencesNational Institute of Environmental Health Science Superfund Research ProgramNIEHS NIH HHS22https://www.ebi.ac.uk/biostudies/studies/S-EPMC9855386biostudies-literatureUnknown13(1)In this study, a previously unreported 3-((4-(isopropylamino)-6-(methylthio)-1,3,5-triazin-2-yl) amino) butyric acid hapten was designed and synthesized. This maximized the exposure of the antigen-determinant isopropyl of prometryn to the immune system in animals to induce the production of anticipated highly specific anti-prometryn antibodies. The hapten has a molecular weight of 285.37 Da. The compound was confirmed by nuclear magnetic resonance hydrogen spectroscopy (<sup>1</sup>H NMR), nuclear magnetic resonance carbon spectroscopy (<sup>13</sup>C NMR), and high-resolution mass spectrometry (HRMS). By using the active ester approach, immunogens and coated antigens were created. Bovine serum albumin (BSA) was used as an immunogen, along with the successfully produced hapten, to immunizeBiosensorsObtaining a Monoclonal Antibody against a Novel Prometryn-Like Hapten and Characterization of Its Selectivity for Triazine Herbicides.PMC9855386Grant No.: Y2021PT05NAS, Subaward No.: 2000009144Y2021PT05Grant No.: P42 ES004699P42 ES0046992000009144Xu LJin FLei XAbd El-Aty AMZhang XJin MCui XShe YZhao JWang JZhao YHammock BDfalseObtaining a Monoclonal Antibody against a Novel Prometryn-Like Hapten and Characterization of Its Selectivity for Triazine Herbicides.In this study, a previously unreported 3-((4-(isopropylamino)-6-(methylthio)-1,3,5-triazin-2-yl) amino) butyric acid hapten was designed and synthesized. This maximized the exposure of the antigen-determinant isopropyl of prometryn to the immune system in animals to induce the production of anticipated highly specific anti-prometryn antibodies. The hapten has a molecular weight of 285.37 Da. The compound was confirmed by nuclear magnetic resonance hydrogen spectroscopy (<sup>1</sup>H NMR), nuclear magnetic resonance carbon spectroscopy (<sup>13</sup>C NMR), and high-resolution mass spectrometry (HRMS). By using the active ester approach, immunogens and coated antigens were created. Bovine serum albumin (BSA) was used as an immunogen, along with the successfully produced hapten, to immunize2022-01-01T00:00:00Z2022 Dec2025-04-22T09:08:25.191Z2025-04-05T22:51:07.077ZS-EPMC98553863667185810.3390/bios13010022