{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Awad K"],"funding":["the Deutsche Forschungsgemeinschaft (DFG)","the German Research Foundation","the Charité—Universitätsmedizin Berlin"],"pagination":["236"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9856377"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(2)"],"pubmed_abstract":["<h4>Background</h4>The underlying pathophysiology of irritable bowel syndrome (IBS) is still unclear. Our aim was to investigate the pathophysiological mechanisms of diarrhea, constipation, and antigen uptake in mixed-type IBS (IBS-M).<h4>Methods</h4>Colonoscopic biopsies were obtained from IBS-M patients. Epithelial transport and barrier function of colonic mucosae were characterized in Ussing chambers using impedance spectroscopy. Mucosal permeability to macromolecules was measured. Western blotting for tight junction (TJ) proteins was performed and their subcellular localization was visualized by confocal microscopy. RNA-sequencing was performed for gene expression and signaling pathway analysis.<h4>Results</h4>In IBS-M, epithelial resistance and ENaC-dependent sodium absorption were un"],"journal":["Cells"],"pubmed_title":["Impaired Intestinal Permeability of Tricellular Tight Junctions in Patients with Irritable Bowel Syndrome with Mixed Bowel Habits (IBS-M)."],"pmcid":["PMC9856377"],"funding_grant_id":["DFG Schu 559/11-5","GRK 2318-2 C1"],"pubmed_authors":["Schulzke JD","Awad K","Barmeyer C","Bucker R","Bojarski C","Lee IM","Nagel O","Schweiger MR"],"additional_accession":[]},"is_claimable":false,"name":"Impaired Intestinal Permeability of Tricellular Tight Junctions in Patients with Irritable Bowel Syndrome with Mixed Bowel Habits (IBS-M).","description":"<h4>Background</h4>The underlying pathophysiology of irritable bowel syndrome (IBS) is still unclear. Our aim was to investigate the pathophysiological mechanisms of diarrhea, constipation, and antigen uptake in mixed-type IBS (IBS-M).<h4>Methods</h4>Colonoscopic biopsies were obtained from IBS-M patients. Epithelial transport and barrier function of colonic mucosae were characterized in Ussing chambers using impedance spectroscopy. Mucosal permeability to macromolecules was measured. Western blotting for tight junction (TJ) proteins was performed and their subcellular localization was visualized by confocal microscopy. RNA-sequencing was performed for gene expression and signaling pathway analysis.<h4>Results</h4>In IBS-M, epithelial resistance and ENaC-dependent sodium absorption were un","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-04-08T12:10:10.202Z","creation":"2025-02-19T02:23:53.083Z"},"accession":"S-EPMC9856377","cross_references":{"pubmed":["36672170"],"doi":["10.3390/cells12020236"]}}