biostudies-literatureBodmer BSGerman Research FoundationEbola-Foresight.Deutsches Zentrum für Infektionsforschung Chica and Heinz Schaller FoundationGerman Center for Infection ResearchFriedrich-Loeffler-InstitutGerman Ministry of Food and Agriculture2164216https://www.ebi.ac.uk/biostudies/studies/S-EPMC9858441biostudies-literatureUnknown12(1)Ebolaviruses cause outbreaks of haemorrhagic fever in Central and West Africa. Some members of this genus such as Ebola virus (EBOV) are highly pathogenic, with case fatality rates of up to 90%, whereas others such as Reston virus (RESTV) are apathogenic for humans. Bombali virus (BOMV) is a novel ebolavirus for which complete genome sequences were recently found in free-tailed bats, although no infectious virus could be isolated. Its pathogenic potential for humans is unknown. To address this question, we first determined whether proteins encoded by the available BOMV sequence found in <i>Chaerephon pumilus</i> were functional in <i>in vitro</i> assays. The correction of an apparent sequencing error in the glycoprotein based on these data then allowed us to generate infectious BOMV using Emerging microbes & infections<i>In vivo</i> characterization of the novel ebolavirus Bombali virus suggests a low pathogenic potential for humans.PMC9858441469065579TTU 01.702Heung MBrunetti JEVallbracht MChlanda PWendt LRomer SBreithaupt ARodriguez EMuller-Guhl JHoenen TBodmer BSWinter SLHenkel CMuller AEscudero-Perez BMunoz-Fontela Cfalse<i>In vivo</i> characterization of the novel ebolavirus Bombali virus suggests a low pathogenic potential for humans.Ebolaviruses cause outbreaks of haemorrhagic fever in Central and West Africa. Some members of this genus such as Ebola virus (EBOV) are highly pathogenic, with case fatality rates of up to 90%, whereas others such as Reston virus (RESTV) are apathogenic for humans. Bombali virus (BOMV) is a novel ebolavirus for which complete genome sequences were recently found in free-tailed bats, although no infectious virus could be isolated. Its pathogenic potential for humans is unknown. To address this question, we first determined whether proteins encoded by the available BOMV sequence found in <i>Chaerephon pumilus</i> were functional in <i>in vitro</i> assays. The correction of an apparent sequencing error in the glycoprotein based on these data then allowed us to generate infectious BOMV using 2023-01-01T00:00:00Z2023 Dec2026-05-02T11:14:25.777Z2024-11-20T12:10:15.622ZS-EPMC98584413658044010.1080/22221751.2022.2164216