biostudies-literaturePeng XDanone Institute (China)National Natural Science Foundation of China111 project404https://www.ebi.ac.uk/biostudies/studies/S-EPMC9858452biostudies-literatureUnknown12(2)In vitro digestion methods that can accurately predict the estimated GI (eGI) values of complex carbohydrate foods, including biscuits, are worth exploring. In the current study, standard commercial biscuits with varied clinical GI values between 9~30 were digested using both the INFOGEST and single-enzyme digestion protocols. The digestion kinetic parameters were acquired through mathematical fitting by mathematical kinetics models. The results showed that compared with the INFOGEST protocol, the AUR180 deduced from digesting using either porcine pancreatin or α-amylase showed the best potential in predicting the eGI values. Accordingly, mathematical equations were established based on the relations between the AUR180 and the GI values. When digesting using porcine pancreatin, GI= 1.834 +Foods (Basel, Switzerland)Predicting the Glycemic Index of Biscuits Using Static In Vitro Digestion Protocols.PMC9858452B17018DIC2021-063227234032101867Peng XLiu HLi XZhang KLi SBao XYu WWang HZou WfalsePredicting the Glycemic Index of Biscuits Using Static In Vitro Digestion Protocols.In vitro digestion methods that can accurately predict the estimated GI (eGI) values of complex carbohydrate foods, including biscuits, are worth exploring. In the current study, standard commercial biscuits with varied clinical GI values between 9~30 were digested using both the INFOGEST and single-enzyme digestion protocols. The digestion kinetic parameters were acquired through mathematical fitting by mathematical kinetics models. The results showed that compared with the INFOGEST protocol, the AUR180 deduced from digesting using either porcine pancreatin or α-amylase showed the best potential in predicting the eGI values. Accordingly, mathematical equations were established based on the relations between the AUR180 and the GI values. When digesting using porcine pancreatin, GI= 1.834 +2023-01-01T00:00:00Z2023 Jan2025-08-16T03:06:34.645Z2025-04-05T20:52:53.823ZS-EPMC98584523667349910.3390/foods12020404