{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sheng Q"],"funding":["Wellcome Trust"],"pagination":["eadd8659"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9858504"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(3)"],"pubmed_abstract":["Braun's lipoprotein (Lpp) plays a major role in stabilizing the integrity of the cell envelope in <i>Escherichia coli</i>, as it provides a covalent cross-link between the outer membrane and the peptidoglycan layer. An important challenge in elucidating the physiological role of Lpp lies in attaining a detailed understanding of its distribution on the peptidoglycan layer. Here, using atomic force microscopy, we visualized Lpp directly on peptidoglycan sacculi. Lpp is homogeneously distributed over the outer surface of the sacculus at a high density. However, it is absent at the constriction site during cell division, revealing its role in the cell division process with Pal, another cell envelope-associated protein. Collectively, we have established a framework to elucidate the distribution"],"journal":["Science advances"],"pubmed_title":["In situ visualization of Braun's lipoprotein on <i>E. coli</i> sacculi."],"pmcid":["PMC9858504"],"funding_grant_id":["212197/Z/19/Z","212197/Z/18/Z"],"pubmed_authors":["Zhang YZ","Su HN","Liu LN","Hobbs JK","Zhang HS","Li K","Liu SM","Liu NH","Chen ZW","Zhang MY","Yang PL","Liu MY","Zhao LS","Sheng Q","Foster SJ","Chen XL"],"additional_accession":[]},"is_claimable":false,"name":"In situ visualization of Braun's lipoprotein on <i>E. coli</i> sacculi.","description":"Braun's lipoprotein (Lpp) plays a major role in stabilizing the integrity of the cell envelope in <i>Escherichia coli</i>, as it provides a covalent cross-link between the outer membrane and the peptidoglycan layer. An important challenge in elucidating the physiological role of Lpp lies in attaining a detailed understanding of its distribution on the peptidoglycan layer. Here, using atomic force microscopy, we visualized Lpp directly on peptidoglycan sacculi. Lpp is homogeneously distributed over the outer surface of the sacculus at a high density. However, it is absent at the constriction site during cell division, revealing its role in the cell division process with Pal, another cell envelope-associated protein. Collectively, we have established a framework to elucidate the distribution","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-05-02T11:13:06.363Z","creation":"2025-02-19T03:27:19.739Z"},"accession":"S-EPMC9858504","cross_references":{"pubmed":["36662863"],"doi":["10.1126/sciadv.add8659"]}}