biostudies-literatureMaier DDeutsche ForschungsgemeinschaftNCI NIH HHSNIH HHSNational Science Foundation205https://www.ebi.ac.uk/biostudies/studies/S-EPMC9858708biostudies-literatureUnknown14(1)Cellular differentiation relies on the highly conserved Notch signaling pathway. Notch activity induces gene expression changes that are highly sensitive to chromatin landscape. We address Notch gene regulation using <i>Drosophila</i> as a model, focusing on the genetic and molecular interactions between the Notch antagonist Hairless and the histone chaperone Asf1. Earlier work implied that Asf1 promotes the silencing of Notch target genes via Hairless (H). Here, we generate a novel <i>H</i>^Δ<i>CT</i> allele by genome engineering. Phenotypically, <i>H</i>^Δ<i>CT</i> behaves as a Hairless gain of function allele in several developmental contexts, indicating that the conserved CT domain of H has an attenuator role under native biological contexts. Using several independeGenesGenetic and Molecular Interactions between <i>H</i>^Δ<i>CT</i>, a Novel Allele of the Notch Antagonist Hairless, and the Histone Chaperone Asf1 in <i>Drosophila melanogaster</i>.PMC9858708NA 427/5-1 and NA 427/6-1 to ACNCA 178974 to RAKMA 1328/11-1 to DMR01 CA178974MCBBSF grant #2114950 to RAKBauer MBoger MScharpf JSanchez Jimenez AKovall RAFechner JMaier DNagel ACPreiss AYuan ZfalseGenetic and Molecular Interactions between <i>H</i>^Δ<i>CT</i>, a Novel Allele of the Notch Antagonist Hairless, and the Histone Chaperone Asf1 in <i>Drosophila melanogaster</i>.Cellular differentiation relies on the highly conserved Notch signaling pathway. Notch activity induces gene expression changes that are highly sensitive to chromatin landscape. We address Notch gene regulation using <i>Drosophila</i> as a model, focusing on the genetic and molecular interactions between the Notch antagonist Hairless and the histone chaperone Asf1. Earlier work implied that Asf1 promotes the silencing of Notch target genes via Hairless (H). Here, we generate a novel <i>H</i>^Δ<i>CT</i> allele by genome engineering. Phenotypically, <i>H</i>^Δ<i>CT</i> behaves as a Hairless gain of function allele in several developmental contexts, indicating that the conserved CT domain of H has an attenuator role under native biological contexts. Using several independe2023-01-01T00:00:00Z2023 Jan2025-04-22T09:05:03.99Z2025-04-05T22:49:29.086ZS-EPMC98587083667294610.3390/genes14010205