biostudies-literatureXu JGuizhou Provincial Science and Technology ProjectGuizhou Provincial Agricultural Major Industrial Scientific Research Project113https://www.ebi.ac.uk/biostudies/studies/S-EPMC9858937biostudies-literatureUnknown14(1)In this study, Xiangsu hybrid pig growth traits were evaluated via <i>PRKAA2</i> and <i>MSMB</i> as candidate genes. Sanger sequencing revealed three mutation sites in <i>PRKAA2</i>, namely, g.42101G>T, g.60146A>T, and g.61455G>A, and all these sites were intronic mutations. Moreover, six mutation sites were identified in <i>MSMB</i>: intronic g.4374G>T, exonic g.4564T>C, exonic g.6378G>A, exonic g.6386C>T, intronic g.8643G>A, and intronic g.8857A>G. Association analysis revealed that g.42101G>T, g.60146A>T, g.61455G>A, g.4374G>T, g.4564T>C, g.6378G>A, g.6386C>T, g.8643G>A, and g.8857A>G showed different relationship patterns among body weight, body length, body height, chest circumference, abdominal circumference, tube circumference, and chest depth. Real-time polymerase chain reaction reGenesAssociation Analysis of <i>PRKAA2</i> and <i>MSMB</i> Polymorphisms and Growth Traits of Xiangsu Hybrid Pigs.PMC9858937QKHFQ-2018,4007, (002)QKHKYZ-2019,011Ruan YDai LXiao MHuang JShi PXu HXu JSun JfalseAssociation Analysis of <i>PRKAA2</i> and <i>MSMB</i> Polymorphisms and Growth Traits of Xiangsu Hybrid Pigs.In this study, Xiangsu hybrid pig growth traits were evaluated via <i>PRKAA2</i> and <i>MSMB</i> as candidate genes. Sanger sequencing revealed three mutation sites in <i>PRKAA2</i>, namely, g.42101G>T, g.60146A>T, and g.61455G>A, and all these sites were intronic mutations. Moreover, six mutation sites were identified in <i>MSMB</i>: intronic g.4374G>T, exonic g.4564T>C, exonic g.6378G>A, exonic g.6386C>T, intronic g.8643G>A, and intronic g.8857A>G. Association analysis revealed that g.42101G>T, g.60146A>T, g.61455G>A, g.4374G>T, g.4564T>C, g.6378G>A, g.6386C>T, g.8643G>A, and g.8857A>G showed different relationship patterns among body weight, body length, body height, chest circumference, abdominal circumference, tube circumference, and chest depth. Real-time polymerase chain reaction re2022-01-01T00:00:00Z2022 Dec2025-04-05T00:16:18.018Z2024-11-05T15:11:21.448ZS-EPMC98589373667285410.3390/genes14010113