{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["18(1)"],"submitter":["Rapino F"],"pubmed_abstract":["In the brain, the complement system plays a crucial role in the immune response and in synaptic elimination during normal development and disease. Here, we sought to identify pathways that modulate the production of complement component 4 (C4), recently associated with an increased risk of schizophrenia. To design a disease-relevant assay, we first developed a rapid and robust 3D protocol capable of producing large numbers of astrocytes from pluripotent cells. Transcriptional profiling of these astrocytes confirmed the homogeneity of this population of dorsal fetal-like astrocytes. Using a novel ELISA-based small-molecule screen, we identified epigenetic regulators, as well as inhibitors of intracellular signaling pathways, able to modulate C4 secretion from astrocytes. We then built a con"],"journal":["Stem cell reports"],"pagination":["237-253"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9860128"],"repository":["biostudies-literature"],"pubmed_title":["Small-molecule screen reveals pathways that regulate C4 secretion in stem cell-derived astrocytes."],"pmcid":["PMC9860128"],"pubmed_authors":["Beattie UK","Norabuena E","Natoli T","Angelini G","Limone F","Hazelbaker D","Subramanian A","Blank J","Thanos JM","de Rivera H","Rubin LL","Tegtmeyer M","McCarroll SA","Narula J","Stevens B","Nehme R","Chen W","Rapino F","Barrett L","O'Neil A","Sheridan SD","Perlis RH","O'Connor E"],"additional_accession":[]},"is_claimable":false,"name":"Small-molecule screen reveals pathways that regulate C4 secretion in stem cell-derived astrocytes.","description":"In the brain, the complement system plays a crucial role in the immune response and in synaptic elimination during normal development and disease. Here, we sought to identify pathways that modulate the production of complement component 4 (C4), recently associated with an increased risk of schizophrenia. To design a disease-relevant assay, we first developed a rapid and robust 3D protocol capable of producing large numbers of astrocytes from pluripotent cells. Transcriptional profiling of these astrocytes confirmed the homogeneity of this population of dorsal fetal-like astrocytes. Using a novel ELISA-based small-molecule screen, we identified epigenetic regulators, as well as inhibitors of intracellular signaling pathways, able to modulate C4 secretion from astrocytes. We then built a con","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-26T00:39:08.408Z","creation":"2025-02-19T03:00:27.188Z"},"accession":"S-EPMC9860128","cross_references":{"pubmed":["36563689"],"doi":["10.1016/j.stemcr.2022.11.018"]}}