biostudies-literatureHaghverdi LNHGRI NIH HHS13-25https://www.ebi.ac.uk/biostudies/studies/S-EPMC9860164biostudies-literatureUnknown18(1)The concept of cell fate relates to the future identity of a cell, and its daughters, which is obtained via cell differentiation and division. Understanding, predicting, and manipulating cell fate has been a long-sought goal of developmental and regenerative biology. Recent insights obtained from single-cell genomic and integrative lineage-tracing approaches have further aided to identify molecular features predictive of cell fate. In this perspective, we discuss these approaches with a focus on theoretical concepts and future directions of the field to dissect molecular mechanisms underlying cell fate.Stem cell reportsSingle-cell multi-omics and lineage tracing to dissect cell fate decision-making.PMC9860164UM1 HG012076Ludwig LSHaghverdi LfalseSingle-cell multi-omics and lineage tracing to dissect cell fate decision-making.The concept of cell fate relates to the future identity of a cell, and its daughters, which is obtained via cell differentiation and division. Understanding, predicting, and manipulating cell fate has been a long-sought goal of developmental and regenerative biology. Recent insights obtained from single-cell genomic and integrative lineage-tracing approaches have further aided to identify molecular features predictive of cell fate. In this perspective, we discuss these approaches with a focus on theoretical concepts and future directions of the field to dissect molecular mechanisms underlying cell fate.2023-01-01T00:00:00Z2023 Jan2026-06-03T18:29:25.909Z2025-04-05T18:36:20.181ZS-EPMC98601643663090010.1016/j.stemcr.2022.12.003