biostudies-literaturePradal ISpanish National Research CouncilGobierno del Principado de Asturias179https://www.ebi.ac.uk/biostudies/studies/S-EPMC9860891biostudies-literatureUnknown15(1)The rise of antimicrobial resistant (AMR) bacteria is a major health concern, especially with regard to members of the ESKAPE group, to which vancomycin-resistant (VRE) <i>Enterococcus faecium</i> belongs. Phage therapy has emerged as a novel alternative for the treatment of AMR infections. This, however, relies on the isolation and characterisation of a large collection of phages. This work describes the exploration of human faeces as a source of new <i>E. faecium</i>-infecting phages. Phage vB_EfaH_163 was isolated and characterised at the microbiological, genomic, and functional levels. vB_EfaH_163 phage, a new member of <i>Herelleviridae</i>, subfamily <i>Brockvirinae</i>, has a dsDNA genome of 150,836 bp that does not harbour any virulence factors or antibiotic resistance genes. It inViruses<i>Enterococcus faecium</i> Bacteriophage vB_EfaH_163, a New Member of the <i>Herelleviridae</i> Family, Reduces the Mortality Associated with an <i>E. faecium</i> vanR Clinical Isolate in a <i>Galleria mellonella</i> Animal Model.PMC9860891AYUD/2021/50916CSIC201870I091Pradal IDel Rio BFernandez MCasado ALadero VRodriguez-Lucas CAlvarez MAfalse<i>Enterococcus faecium</i> Bacteriophage vB_EfaH_163, a New Member of the <i>Herelleviridae</i> Family, Reduces the Mortality Associated with an <i>E. faecium</i> vanR Clinical Isolate in a <i>Galleria mellonella</i> Animal Model.The rise of antimicrobial resistant (AMR) bacteria is a major health concern, especially with regard to members of the ESKAPE group, to which vancomycin-resistant (VRE) <i>Enterococcus faecium</i> belongs. Phage therapy has emerged as a novel alternative for the treatment of AMR infections. This, however, relies on the isolation and characterisation of a large collection of phages. This work describes the exploration of human faeces as a source of new <i>E. faecium</i>-infecting phages. Phage vB_EfaH_163 was isolated and characterised at the microbiological, genomic, and functional levels. vB_EfaH_163 phage, a new member of <i>Herelleviridae</i>, subfamily <i>Brockvirinae</i>, has a dsDNA genome of 150,836 bp that does not harbour any virulence factors or antibiotic resistance genes. It in2023-01-01T00:00:00Z2023 Jan2026-06-28T03:08:50.357Z2024-11-15T19:59:11.095ZS-EPMC98608913668021910.3390/v15010179