{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["28(2)"],"submitter":["Wang X"],"pubmed_abstract":["A series of seven novel iridium complexes were synthetized and characterized as potential photosensitizers for photodynamic therapy (PDT) applications. Among them, four complexes were evaluated in vitro for their anti-proliferative activity with and without irradiation on a panel of five cancer cell lines, namely PC-3 (prostate cancer), T24 (bladder cancer), MCF7 (breast cancer), A549 (lung cancer) and HeLa (cervix cancer), and two non-cancerous cell models (NIH-3T3 fibroblasts and MC3T3 osteoblasts). After irradiation at 458 nm, all tested complexes showed a strong selectivity against cancer cells, with a selectivity index (SI) ranging from 8 to 34 compared with non-cancerous cells. The cytotoxic effect of all these complexes was found to be independent of the anti-apoptotic protein Bcl-xL. The compound exhibiting the best selectivity, complex <b>4a</b>, was selected for further investigations. Complex <b>4a</b> was mainly localized in the mitochondria. We found that the loss of cell viability and the decrease in ATP and GSH content induced by complex <b>4a</b> were independent of both Bcl-xL and caspase activation, leading to a non-apoptotic cell death. By counteracting the intrinsic or acquired resistance to apoptosis associated with cancer, complex <b>4a</b> could be an interesting therapeutic alternative to be studied in preclinical models."],"journal":["Molecules (Basel, Switzerland)"],"pagination":["691"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9861386"],"repository":["biostudies-literature"],"pubmed_title":["<i>N</i>-Heterocyclic Carbene-Iridium Complexes as Photosensitizers for In Vitro Photodynamic Therapy to Trigger Non-Apoptotic Cell Death in Cancer Cells."],"pmcid":["PMC9861386"],"pubmed_authors":["Deraeve C","Gornitzka H","Bijani C","Cuvillier O","Madji R","Wang X","Mazeres S","Zhang C","Voros C","Maddelein ML","Hemmert C"],"additional_accession":[]},"is_claimable":false,"name":"<i>N</i>-Heterocyclic Carbene-Iridium Complexes as Photosensitizers for In Vitro Photodynamic Therapy to Trigger Non-Apoptotic Cell Death in Cancer Cells.","description":"A series of seven novel iridium complexes were synthetized and characterized as potential photosensitizers for photodynamic therapy (PDT) applications. Among them, four complexes were evaluated in vitro for their anti-proliferative activity with and without irradiation on a panel of five cancer cell lines, namely PC-3 (prostate cancer), T24 (bladder cancer), MCF7 (breast cancer), A549 (lung cancer) and HeLa (cervix cancer), and two non-cancerous cell models (NIH-3T3 fibroblasts and MC3T3 osteoblasts). After irradiation at 458 nm, all tested complexes showed a strong selectivity against cancer cells, with a selectivity index (SI) ranging from 8 to 34 compared with non-cancerous cells. The cytotoxic effect of all these complexes was found to be independent of the anti-apoptotic protein Bcl-xL. The compound exhibiting the best selectivity, complex <b>4a</b>, was selected for further investigations. Complex <b>4a</b> was mainly localized in the mitochondria. We found that the loss of cell viability and the decrease in ATP and GSH content induced by complex <b>4a</b> were independent of both Bcl-xL and caspase activation, leading to a non-apoptotic cell death. By counteracting the intrinsic or acquired resistance to apoptosis associated with cancer, complex <b>4a</b> could be an interesting therapeutic alternative to be studied in preclinical models.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-04T11:33:32.597Z","creation":"2025-04-04T11:33:32.597Z"},"accession":"S-EPMC9861386","cross_references":{"pubmed":["36677751"],"doi":["10.3390/molecules28020691"]}}