biostudies-literatureZhang QNatural Science Foundation of Shandong ProvinceShandong First Medical UniversityTaishan Scholar Foundation of Shandong Province58https://www.ebi.ac.uk/biostudies/studies/S-EPMC9862811biostudies-literatureUnknown18(1)<h4>Background</h4>Osteoarthritis is a chronic degenerative joint disease, and increasing evidences suggest that the pathogenic mechanism involves immune system and inflammation.<h4>Aims</h4>The aim of current study was to uncover hub genes linked to immune infiltration in osteoarthritis synovial tissue using comprehensive bioinformatics analysis and experimental confirmation.<h4>Methods</h4>Multiple microarray datasets (GSE55457, GSE55235, GSE12021 and GSE1919) for osteoarthritis in Gene Expression Omnibus database were downloaded for analysis. Differentially expressed genes (DEGs) were identified using Limma package in R software, and immune infiltration was evaluated by CIBERSORT algorithm. Then weighted gene co-expression network analysis (WGCNA) was performed to uncover immune infiltrJournal of orthopaedic surgery and researchMechanism of immune infiltration in synovial tissue of osteoarthritis: a gene expression-based study.PMC98628112020RC008tsqn201909183ZR2020QH072Liu XMa CSun CFeng RZhang QZhu CfalseMechanism of immune infiltration in synovial tissue of osteoarthritis: a gene expression-based study.<h4>Background</h4>Osteoarthritis is a chronic degenerative joint disease, and increasing evidences suggest that the pathogenic mechanism involves immune system and inflammation.<h4>Aims</h4>The aim of current study was to uncover hub genes linked to immune infiltration in osteoarthritis synovial tissue using comprehensive bioinformatics analysis and experimental confirmation.<h4>Methods</h4>Multiple microarray datasets (GSE55457, GSE55235, GSE12021 and GSE1919) for osteoarthritis in Gene Expression Omnibus database were downloaded for analysis. Differentially expressed genes (DEGs) were identified using Limma package in R software, and immune infiltration was evaluated by CIBERSORT algorithm. Then weighted gene co-expression network analysis (WGCNA) was performed to uncover immune infiltr2023-01-01T00:00:00Z2023 Jan2026-06-20T03:12:21.713Z2025-04-05T20:53:02.905ZS-EPMC98628113668183710.1186/s13018-023-03541-x