biostudies-literatureHoene MGerman Diabetes AssociationUniversity of TübingenGerman Center for Diabetes ResearchSino-German Center for Research PromotionOpen Access Publishing Fund of the University of TübingenNational Natural Science Foundation of ChinaFederal Ministry of Education and ResearchGerman Federal Ministry of Education and Research15https://www.ebi.ac.uk/biostudies/studies/S-EPMC9863672biostudies-literatureUnknown13(1)Physical exercise is a powerful measure to prevent cardiometabolic diseases. However, the individual response to lifestyle interventions is variable and cannot, to date, be predicted. N-Lactoylphenylalanine (Lac-Phe) produced during exercise has recently been shown to mediate weight loss in obese mice. Lac-Phe could also contribute to, and potentially explain differences in, the effectiveness of exercise interventions in humans. Sedentary overweight and obese subjects completed an 8-week supervised endurance exercise intervention (n = 22). Before and after the intervention, plasma levels of Lac-Phe were determined by UHPLC-MS in the resting state and immediately after an acute bout of endurance exercise. Adipose tissue volume was quantified using MRI. Acute exercise caused a pronounced incMetabolitesExercise-Induced N-Lactoylphenylalanine Predicts Adipose Tissue Loss during Endurance Training in Overweight and Obese Humans.PMC9863672M-025701GI092521934006n.a.Machann JWeigert CPeter ANiess AHeni MMoller AXu GHoene MZhao XBirkenfeld ALLehmann RfalseExercise-Induced N-Lactoylphenylalanine Predicts Adipose Tissue Loss during Endurance Training in Overweight and Obese Humans.Physical exercise is a powerful measure to prevent cardiometabolic diseases. However, the individual response to lifestyle interventions is variable and cannot, to date, be predicted. N-Lactoylphenylalanine (Lac-Phe) produced during exercise has recently been shown to mediate weight loss in obese mice. Lac-Phe could also contribute to, and potentially explain differences in, the effectiveness of exercise interventions in humans. Sedentary overweight and obese subjects completed an 8-week supervised endurance exercise intervention (n = 22). Before and after the intervention, plasma levels of Lac-Phe were determined by UHPLC-MS in the resting state and immediately after an acute bout of endurance exercise. Adipose tissue volume was quantified using MRI. Acute exercise caused a pronounced inc2022-01-01T00:00:00Z2022 Dec2025-04-04T10:19:48.15Z2025-02-19T00:12:25.399ZS-EPMC98636723667694010.3390/metabo13010015