{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li S"],"funding":["National Natural Science Foundation of China","the Central Public-interest Scientific Institution Basal Research Fund"],"pagination":["160"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9863715"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["<i>Streptococcus suis</i> is an important swine bacterial pathogen that activates macrophages to secrete inflammatory cytokines. Primary porcine alveolar macrophages (PAMs) are inconvenient to obtain, but it is unknown whether immortalized PAM-Tang cells can replace them as a better cell model for the study of the interaction between <i>S. suis</i> and macrophages. In this study, the phagocytic integrity, polarization, and pro-inflammatory cytokine secretion of PAM-Tang cells were confirmed by live-cell imaging, electron microscopy, confocal microscopy, and ELISA. Interestingly, the <i>S. suis</i> serotype 9 avirulent strain W7119 induced higher levels of adhesion and pro-inflammatory cytokines in PAM-Tang cells than the <i>S. suis</i> serotype 2 virulent strain 700794. Prolonged incubatio"],"journal":["Microorganisms"],"pubmed_title":["Interaction between Porcine Alveolar Macrophage-Tang Cells and <i>Streptococcus suis</i> Strains of Different Virulence:&amp;nbsp;Phagocytosis and Apoptosis."],"pmcid":["PMC9863715"],"funding_grant_id":["1610302022006","32273018"],"pubmed_authors":["Li S","Qin L","Wang C","Wang S","Tang YD","Bai Y","Cai X","Chen T"],"additional_accession":[]},"is_claimable":false,"name":"Interaction between Porcine Alveolar Macrophage-Tang Cells and <i>Streptococcus suis</i> Strains of Different Virulence:&amp;nbsp;Phagocytosis and Apoptosis.","description":"<i>Streptococcus suis</i> is an important swine bacterial pathogen that activates macrophages to secrete inflammatory cytokines. Primary porcine alveolar macrophages (PAMs) are inconvenient to obtain, but it is unknown whether immortalized PAM-Tang cells can replace them as a better cell model for the study of the interaction between <i>S. suis</i> and macrophages. In this study, the phagocytic integrity, polarization, and pro-inflammatory cytokine secretion of PAM-Tang cells were confirmed by live-cell imaging, electron microscopy, confocal microscopy, and ELISA. Interestingly, the <i>S. suis</i> serotype 9 avirulent strain W7119 induced higher levels of adhesion and pro-inflammatory cytokines in PAM-Tang cells than the <i>S. suis</i> serotype 2 virulent strain 700794. Prolonged incubatio","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-22T01:07:27.538Z","creation":"2025-04-05T19:49:40.613Z"},"accession":"S-EPMC9863715","cross_references":{"pubmed":["36677452"],"doi":["10.3390/microorganisms11010160"]}}