biostudies-literatureLi SNational Natural Science Foundation of Chinathe Central Public-interest Scientific Institution Basal Research Fund160https://www.ebi.ac.uk/biostudies/studies/S-EPMC9863715biostudies-literatureUnknown11(1)Streptococcus suis is an important swine bacterial pathogen that activates macrophages to secrete inflammatory cytokines. Primary porcine alveolar macrophages (PAMs) are inconvenient to obtain, but it is unknown whether immortalized PAM-Tang cells can replace them as a better cell model for the study of the interaction between S. suis and macrophages. In this study, the phagocytic integrity, polarization, and pro-inflammatory cytokine secretion of PAM-Tang cells were confirmed by live-cell imaging, electron microscopy, confocal microscopy, and ELISA. Interestingly, the S. suis serotype 9 avirulent strain W7119 induced higher levels of adhesion and pro-inflammatory cytokines in PAM-Tang cells than the S. suis serotype 2 virulent strain 700794. Prolonged incubatioMicroorganismsInteraction between Porcine Alveolar Macrophage-Tang Cells and Streptococcus suis Strains of Different Virulence:&nbsp;Phagocytosis and Apoptosis.PMC9863715161030202200632273018Li SQin LWang CWang STang YDBai YCai XChen TfalseInteraction between Porcine Alveolar Macrophage-Tang Cells and Streptococcus suis Strains of Different Virulence:&nbsp;Phagocytosis and Apoptosis.Streptococcus suis is an important swine bacterial pathogen that activates macrophages to secrete inflammatory cytokines. Primary porcine alveolar macrophages (PAMs) are inconvenient to obtain, but it is unknown whether immortalized PAM-Tang cells can replace them as a better cell model for the study of the interaction between S. suis and macrophages. In this study, the phagocytic integrity, polarization, and pro-inflammatory cytokine secretion of PAM-Tang cells were confirmed by live-cell imaging, electron microscopy, confocal microscopy, and ELISA. Interestingly, the S. suis serotype 9 avirulent strain W7119 induced higher levels of adhesion and pro-inflammatory cytokines in PAM-Tang cells than the S. suis serotype 2 virulent strain 700794. Prolonged incubatio2023-01-01T00:00:00Z2023 Jan2025-04-22T01:07:27.538Z2025-04-05T19:49:40.613ZS-EPMC98637153667745210.3390/microorganisms11010160