{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Feringa FM"],"funding":["European Union ERC advanced grant","Lundbeck Foundation"],"pagination":["347-358"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9864589"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["43(3)"],"pubmed_abstract":["The presynaptic proteins MUNC18-1, syntaxin-1, and SNAP25 drive SNARE-mediated synaptic vesicle fusion and are also required for neuronal viability. Their absence triggers rapid, cell-autonomous, neuron-specific degeneration, unrelated to synaptic vesicle deficits. The underlying cell death pathways remain poorly understood. Here, we show that hippocampi of <i>munc18-1</i> null mice (unknown sex) express apoptosis hallmarks cleaved caspase 3 (CC-3) and phosphorylated p53, and have condensed nuclei. However, side-by-side <i>in vitro</i> comparison with classical apoptosis induced by camptothecin uncovered striking differences to syntaxin-1 and MUNC18-1 depleted neurons. First, live-cell imaging revealed consecutive neurite retraction hours before cell death in MUNC18-1 or syntaxin-1 deplete"],"journal":["The Journal of neuroscience : the official journal of the Society for Neuroscience"],"pubmed_title":["An Atypical, Staged Cell Death Pathway Induced by Depletion of SNARE-Proteins MUNC18-1 or Syntaxin-1."],"pmcid":["PMC9864589"],"funding_grant_id":["R277-2018-802","322966"],"pubmed_authors":["van Berkel AA","Feringa FM","Verhage M","Nair A"],"additional_accession":[]},"is_claimable":false,"name":"An Atypical, Staged Cell Death Pathway Induced by Depletion of SNARE-Proteins MUNC18-1 or Syntaxin-1.","description":"The presynaptic proteins MUNC18-1, syntaxin-1, and SNAP25 drive SNARE-mediated synaptic vesicle fusion and are also required for neuronal viability. Their absence triggers rapid, cell-autonomous, neuron-specific degeneration, unrelated to synaptic vesicle deficits. The underlying cell death pathways remain poorly understood. Here, we show that hippocampi of <i>munc18-1</i> null mice (unknown sex) express apoptosis hallmarks cleaved caspase 3 (CC-3) and phosphorylated p53, and have condensed nuclei. However, side-by-side <i>in vitro</i> comparison with classical apoptosis induced by camptothecin uncovered striking differences to syntaxin-1 and MUNC18-1 depleted neurons. First, live-cell imaging revealed consecutive neurite retraction hours before cell death in MUNC18-1 or syntaxin-1 deplete","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-22T01:07:55.845Z","creation":"2025-04-05T19:49:49.959Z"},"accession":"S-EPMC9864589","cross_references":{"pubmed":["36517239"],"doi":["10.1523/JNEUROSCI.0611-22.2022"]}}