{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(1)"],"submitter":["Rocha OB"],"pubmed_abstract":["<h4>Background</h4>Paracoccidioidomycosis is a systemic mycosis caused by the inhalation of conidia of the genus <i>Paracoccidioides</i>. During the infectious process, fungal cells use several carbon sources, leading to the production of propionyl-CoA. The latter is metabolized by the methylcitrate synthase, a key enzyme of the methylcitrate cycle. We identified an inhibitor compound (ZINC08964784) that showed antifungal activity against <i>P. brasiliensis</i>.<h4>Methods</h4>This work aimed to understand the fungal metabolic response of <i>P. brasiliensis</i> cells exposed to ZINC08964784 through a proteomics approach. We used a glucose-free medium supplemented with propionate in order to simulate the environment found by the pathogen during the infection. We performed pyruvate dosage, p"],"journal":["Journal of fungi (Basel, Switzerland)"],"pagination":["108"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9865517"],"repository":["biostudies-literature"],"pubmed_title":["New Methylcitrate Synthase Inhibitor Induces Proteolysis, Lipid Degradation and Pyruvate Excretion in <i>Paracoccidioides brasiliensis</i>."],"pmcid":["PMC9865517"],"pubmed_authors":["Freitas E Silva KS","Soares CMA","Bailao AM","Assuncao LDP","Santos TG","Pereira M","Rocha OB","Moraes D"],"additional_accession":[]},"is_claimable":false,"name":"New Methylcitrate Synthase Inhibitor Induces Proteolysis, Lipid Degradation and Pyruvate Excretion in <i>Paracoccidioides brasiliensis</i>.","description":"<h4>Background</h4>Paracoccidioidomycosis is a systemic mycosis caused by the inhalation of conidia of the genus <i>Paracoccidioides</i>. During the infectious process, fungal cells use several carbon sources, leading to the production of propionyl-CoA. The latter is metabolized by the methylcitrate synthase, a key enzyme of the methylcitrate cycle. We identified an inhibitor compound (ZINC08964784) that showed antifungal activity against <i>P. brasiliensis</i>.<h4>Methods</h4>This work aimed to understand the fungal metabolic response of <i>P. brasiliensis</i> cells exposed to ZINC08964784 through a proteomics approach. We used a glucose-free medium supplemented with propionate in order to simulate the environment found by the pathogen during the infection. We performed pyruvate dosage, p","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-05-31T23:31:07.154Z","creation":"2025-05-31T23:31:07.154Z"},"accession":"S-EPMC9865517","cross_references":{"pubmed":["36675929"],"doi":["10.3390/jof9010108"]}}