biostudies-literatureUnknown9(1)Rocha OB<h4>Background</h4>Paracoccidioidomycosis is a systemic mycosis caused by the inhalation of conidia of the genus <i>Paracoccidioides</i>. During the infectious process, fungal cells use several carbon sources, leading to the production of propionyl-CoA. The latter is metabolized by the methylcitrate synthase, a key enzyme of the methylcitrate cycle. We identified an inhibitor compound (ZINC08964784) that showed antifungal activity against <i>P. brasiliensis</i>.<h4>Methods</h4>This work aimed to understand the fungal metabolic response of <i>P. brasiliensis</i> cells exposed to ZINC08964784 through a proteomics approach. We used a glucose-free medium supplemented with propionate in order to simulate the environment found by the pathogen during the infection. We performed pyruvate dosage, pJournal of fungi (Basel, Switzerland)108https://www.ebi.ac.uk/biostudies/studies/S-EPMC9865517biostudies-literatureNew Methylcitrate Synthase Inhibitor Induces Proteolysis, Lipid Degradation and Pyruvate Excretion in <i>Paracoccidioides brasiliensis</i>.PMC9865517Freitas E Silva KSSoares CMABailao AMAssuncao LDPSantos TGPereira MRocha OBMoraes DfalseNew Methylcitrate Synthase Inhibitor Induces Proteolysis, Lipid Degradation and Pyruvate Excretion in <i>Paracoccidioides brasiliensis</i>.<h4>Background</h4>Paracoccidioidomycosis is a systemic mycosis caused by the inhalation of conidia of the genus <i>Paracoccidioides</i>. During the infectious process, fungal cells use several carbon sources, leading to the production of propionyl-CoA. The latter is metabolized by the methylcitrate synthase, a key enzyme of the methylcitrate cycle. We identified an inhibitor compound (ZINC08964784) that showed antifungal activity against <i>P. brasiliensis</i>.<h4>Methods</h4>This work aimed to understand the fungal metabolic response of <i>P. brasiliensis</i> cells exposed to ZINC08964784 through a proteomics approach. We used a glucose-free medium supplemented with propionate in order to simulate the environment found by the pathogen during the infection. We performed pyruvate dosage, p2023-01-01T00:00:00Z2023 Jan2025-05-31T23:31:07.154Z2025-05-31T23:31:07.154ZS-EPMC98655173667592910.3390/jof9010108