biostudies-literatureFounta KMState Scholarships FoundationHellenic Foundation fo Research and InnovationFondation Santé1163https://www.ebi.ac.uk/biostudies/studies/S-EPMC9865658biostudies-literatureUnknown24(2)Worldwide, there is a great gap between the demand and supply of organs for transplantations. Organs generated from the patients' cells would not only solve the problem of transplant availability but also overcome the complication of incompatibility and tissue rejection by the host immune system. One of the most promising methods tested for the production of organs in vivo is blastocyst complementation (BC). Regrettably, BC is not suitable for the creation of hearts. We have developed a novel method, induced blastocyst complementation (iBC), to surpass this shortcoming. By applying iBC, we generated chimeric mouse embryos, made up of "host" and "donor" cells. We used a specific cardiac enhancer to drive the expression of the diphtheria toxin gene (<i>dtA</i>) in the "host" cells, so that tInternational journal of molecular sciencesDevelopment of a Method for the In Vivo Generation of Allogeneic Hearts in Chimeric Mouse Embryos.PMC986565836528022019MIS-5000432Papanayotou CKanaki ZFounta KMTourkodimitri MIBisti SfalseDevelopment of a Method for the In Vivo Generation of Allogeneic Hearts in Chimeric Mouse Embryos.Worldwide, there is a great gap between the demand and supply of organs for transplantations. Organs generated from the patients' cells would not only solve the problem of transplant availability but also overcome the complication of incompatibility and tissue rejection by the host immune system. One of the most promising methods tested for the production of organs in vivo is blastocyst complementation (BC). Regrettably, BC is not suitable for the creation of hearts. We have developed a novel method, induced blastocyst complementation (iBC), to surpass this shortcoming. By applying iBC, we generated chimeric mouse embryos, made up of "host" and "donor" cells. We used a specific cardiac enhancer to drive the expression of the diphtheria toxin gene (<i>dtA</i>) in the "host" cells, so that t2023-01-01T00:00:00Z2023 Jan2025-05-29T20:02:40.326Z2024-11-06T14:21:59.324ZS-EPMC98656583667467510.3390/ijms24021163