{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen R"],"funding":["Fundamental Research Funds for the Central Universities","Natural Science Foundation of Liaoning Province of China","National Natural Science Foundation of China"],"pagination":["115"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9866645"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Lipid transporters synergistically contribute to oil accumulation under normal conditions in microalgae; however, their effects on lipid metabolism under stress conditions are unknown. Here, we examined the effect of the co-expression of lipid transporters, fatty acid transporters, (FAX1 and FAX2) and ABC transporter (ABCA2) on lipid metabolism and physiological changes in the green microalga <i>Chlamydomonas</i> under nitrogen (N) starvation. The results showed that the TAG content in <i>FAX1-FAX2-ABCA2</i> over-expressor (OE) was 2.4-fold greater than in the parental line. Notably, in <i>FAX1-FAX2-ABCA2-OE</i>, the major membrane lipids and the starch and cellular biomass content also significantly increased compared with the control lines. Moreover, the expression levels of genes direct"],"journal":["Metabolites"],"pubmed_title":["Co-Expression of Lipid Transporters Simultaneously Enhances Oil and Starch Accumulation in the Green Microalga <i>Chlamydomonas reinhardtii</i> under Nitrogen Starvation."],"pmcid":["PMC9866645"],"funding_grant_id":["DUT21LK14","2020-MS-102","31900221"],"pubmed_authors":["Xue S","Feng Y","Kong F","Yamaoka Y","Chen R","Chi Z"],"additional_accession":[]},"is_claimable":false,"name":"Co-Expression of Lipid Transporters Simultaneously Enhances Oil and Starch Accumulation in the Green Microalga <i>Chlamydomonas reinhardtii</i> under Nitrogen Starvation.","description":"Lipid transporters synergistically contribute to oil accumulation under normal conditions in microalgae; however, their effects on lipid metabolism under stress conditions are unknown. Here, we examined the effect of the co-expression of lipid transporters, fatty acid transporters, (FAX1 and FAX2) and ABC transporter (ABCA2) on lipid metabolism and physiological changes in the green microalga <i>Chlamydomonas</i> under nitrogen (N) starvation. The results showed that the TAG content in <i>FAX1-FAX2-ABCA2</i> over-expressor (OE) was 2.4-fold greater than in the parental line. Notably, in <i>FAX1-FAX2-ABCA2-OE</i>, the major membrane lipids and the starch and cellular biomass content also significantly increased compared with the control lines. Moreover, the expression levels of genes direct","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-04T18:53:34.53Z","creation":"2025-04-04T18:53:34.53Z"},"accession":"S-EPMC9866645","cross_references":{"pubmed":["36677040"],"doi":["10.3390/metabo13010115"]}}