biostudies-literatureJeong MHthe Ministry of Science and ICTCommercialization Promotion Agency for R&D Outcomes (COMPA) and the Korea Medical Device Development Fund grant funded by the Korea government (the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health & Welfare,1006https://www.ebi.ac.uk/biostudies/studies/S-EPMC9867480biostudies-literatureUnknown24(2)Three-dimensional (3D) culture platforms have been adopted in a high-throughput screening (HTS) system to mimic in vivo physiological microenvironments. The automated dispenser has been established commercially to enable spotting or distributing non-viscous or viscous biomaterials onto microplates. However, there are still challenges to the precise and accurate dispensation of cells embedded in hydrogels such as Alginate- and Matrigel-extracellular matrices. We developed and improved an automated contact-free dispensing machine, the ASFA SPOTTER (V5 and V6), which is compatible with 96- and 384-pillar/well plates and 330- and 532-micropillar/well chips for the support of 3D spheroid/organoid models using bioprinting techniques. This enables the distribution of non-viscous and viscous biosaInternational journal of molecular sciencesAn Automated High-Throughput Screening (HTS) Spotter for 3D Tumor Spheroid Formation.PMC9867480(2022)ERIC_07_1KMDF_PR_20200901_0153Park KJeon SYKim JEKim ILee DWJeong MHPark KRKu BfalseAn Automated High-Throughput Screening (HTS) Spotter for 3D Tumor Spheroid Formation.Three-dimensional (3D) culture platforms have been adopted in a high-throughput screening (HTS) system to mimic in vivo physiological microenvironments. The automated dispenser has been established commercially to enable spotting or distributing non-viscous or viscous biomaterials onto microplates. However, there are still challenges to the precise and accurate dispensation of cells embedded in hydrogels such as Alginate- and Matrigel-extracellular matrices. We developed and improved an automated contact-free dispensing machine, the ASFA SPOTTER (V5 and V6), which is compatible with 96- and 384-pillar/well plates and 330- and 532-micropillar/well chips for the support of 3D spheroid/organoid models using bioprinting techniques. This enables the distribution of non-viscous and viscous biosa2023-01-01T00:00:00Z2023 Jan2025-04-26T00:39:48.301Z2025-04-06T09:48:05.107ZS-EPMC98674803667452310.3390/ijms24021006