biostudies-literatureUnknown10Sun XThe main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes <i>via</i> one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N₂ sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid anFrontiers in bioengineering and biotechnology1124450https://www.ebi.ac.uk/biostudies/studies/S-EPMC9868447biostudies-literatureOne-pot encapsulation of lactate dehydrogenase and Fe₃O₄ nanoparticles into a metal-organic framework: A novel magnetic recyclable biocatalyst for the synthesis of D-phenyllactic acid.PMC9868447Hu JHuang HFu YLuo XSun XWang YfalseOne-pot encapsulation of lactate dehydrogenase and Fe₃O₄ nanoparticles into a metal-organic framework: A novel magnetic recyclable biocatalyst for the synthesis of D-phenyllactic acid.The main challenges in bio-catalysis of d-phenyllactic acid (D-PLA) are poor tolerance of lactate dehydrogenase (LDH) to harsh environmental conditions and inability to recycle the catalyst. A novel magnetic framework composite was prepared as solid support for the immobilization of enzymes <i>via</i> one-pot encapsulation in this study. LDH/MNPs@MAF-7 was synthesized by the one-pot encapsulation of both LDH and magnetic nanoparticles (MNPs) in MAF-7. The LDH/MNPs@MAF-7 showed stable biological activity for the efficient biosynthesis of D-PLA. The structure and morphology of LDH/MNPs@MAF-7 were systematically characterized by SEM, FT-IR, XRD, VSM, XPS, TGA and N₂ sorption. These indicated that LDH/MNPs@MAF-7 was successfully synthesized, exhibiting enhanced resistance to acid an2022-01-01T00:00:00Z20222025-04-18T12:00:33.949Z2024-10-15T07:41:50.637ZS-EPMC98684473669863910.3389/fbioe.2022.1124450