{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Sato Y"],"pubmed_abstract":["Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined neuronal networks possessing a modular architecture on high-density microelectrode arrays (HD-MEAs), a state-of-the-art electrophysiological tool for recording neural activity with high spatial and temporal resolutions. We first established a surface coating protocol using a cell-permissive hydrogel to stably attach a polydimethylsiloxane microfluidic film on the HD-MEA. We then recorded the spontaneous neural activity of the engineered neuronal network, which revealed an important portrait of the engineered neuronal network-modular architecture enhances functional complexity by reducing the excessive neural correlation between spatially segregated modules. The results of this study highlight the impact of HD-MEA recordings combined with cell engineering technologies as a novel tool in neuroscience to constructively assess the structure-function relationships in neuronal networks."],"journal":["Frontiers in neuroscience"],"pagination":["943310"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9868575"],"repository":["biostudies-literature"],"pubmed_title":["Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks."],"pmcid":["PMC9868575"],"pubmed_authors":["Kato H","Sato S","Hirano-Iwata A","Yamamoto H","Tanii T","Sato Y"],"additional_accession":[]},"is_claimable":false,"name":"Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks.","description":"Neuronal networks in dissociated culture combined with cell engineering technology offer a pivotal platform to constructively explore the relationship between structure and function in living neuronal networks. Here, we fabricated defined neuronal networks possessing a modular architecture on high-density microelectrode arrays (HD-MEAs), a state-of-the-art electrophysiological tool for recording neural activity with high spatial and temporal resolutions. We first established a surface coating protocol using a cell-permissive hydrogel to stably attach a polydimethylsiloxane microfluidic film on the HD-MEA. We then recorded the spontaneous neural activity of the engineered neuronal network, which revealed an important portrait of the engineered neuronal network-modular architecture enhances functional complexity by reducing the excessive neural correlation between spatially segregated modules. The results of this study highlight the impact of HD-MEA recordings combined with cell engineering technologies as a novel tool in neuroscience to constructively assess the structure-function relationships in neuronal networks.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-04T19:47:47.061Z","creation":"2025-04-04T19:47:47.061Z"},"accession":"S-EPMC9868575","cross_references":{"pubmed":["36699522"],"doi":["10.3389/fnins.2022.943310"]}}