{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Li J"],"pubmed_abstract":["<b>Background:</b> Induced pluripotent stem cells-derived exosomes (iPSCs-Exo) can effectively treat spinal cord injury (SCI) in mice. But the role of iPSCs-Exo in SCI mice and its molecular mechanisms remain unclear. This research intended to study the effects and molecular mechanism of iPSCs-Exo in SCI mice models. <b>Methods:</b> The feature of iPSCs-Exo was determined by transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and western blot. The effects of iPSCs-Exo in the SCI mice model were evaluated by Basso Mouse Scale (BMS) scores and H&E staining. The roles of iPSCs-Exo and miR-199b-5p in LPS-treated BMDM were verified by immunofluorescence, RT-qPCR, and Cytokine assays. The target genes of miR-199b-5p were identified, and the function of miR-199b-5p and i"],"journal":["Frontiers in pharmacology"],"pagination":["1078761"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9871459"],"repository":["biostudies-literature"],"pubmed_title":["Induced pluripotent stem cells as natural biofactories for exosomes carrying miR-199b-5p in the treatment of spinal cord injury."],"pmcid":["PMC9871459"],"pubmed_authors":["Jing Y","Ali F","Li J","Jia B","Jia Y","Yan Y","Wu Y","Bai F","Wang L","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"Induced pluripotent stem cells as natural biofactories for exosomes carrying miR-199b-5p in the treatment of spinal cord injury.","description":"<b>Background:</b> Induced pluripotent stem cells-derived exosomes (iPSCs-Exo) can effectively treat spinal cord injury (SCI) in mice. But the role of iPSCs-Exo in SCI mice and its molecular mechanisms remain unclear. This research intended to study the effects and molecular mechanism of iPSCs-Exo in SCI mice models. <b>Methods:</b> The feature of iPSCs-Exo was determined by transmission electron microscope (TEM), nanoparticle tracking analysis (NTA), and western blot. The effects of iPSCs-Exo in the SCI mice model were evaluated by Basso Mouse Scale (BMS) scores and H&E staining. The roles of iPSCs-Exo and miR-199b-5p in LPS-treated BMDM were verified by immunofluorescence, RT-qPCR, and Cytokine assays. The target genes of miR-199b-5p were identified, and the function of miR-199b-5p and i","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-22T06:18:52.254Z","creation":"2025-04-05T21:41:26.357Z"},"accession":"S-EPMC9871459","cross_references":{"pubmed":["36703756"],"doi":["10.3389/fphar.2022.1078761"]}}