biostudies-literatureBaleeiro FCFBundesministerium für Bildung und ForschungHelmholtz-GemeinschaftCoordenação de Aperfeiçoamento de Pessoal de Nível Superior322-336https://www.ebi.ac.uk/biostudies/studies/S-EPMC9871530biostudies-literatureUnknown16(2)Feeding microbial communities with both organic and inorganic substrates can improve sustainability and feasibility of chain elongation processes. Sustainably produced H₂ , CO₂ , and CO can be co-fed to microorganisms as a source for acetyl-CoA, while a small amount of an ATP-generating organic substrate helps overcome the kinetic hindrances associated with autotrophic carboxylate production. Here, we operated two semi-continuous bioreactor systems with continuous recirculation of H₂ , CO₂ , and CO while co-feeding an organic model feedstock (lactate and acetate) to understand how a mixotrophic community is shaped during carboxylate production. Contrary to the assumption that H₂ , CO₂ , and CO support chain elongation via etMicrobial biotechnologyMixotrophic chain elongation with syngas and lactate as electron donors.PMC987153088887.163504/2018‐0001DQ1701688887.163504/2018-00Kleinsteuber SStrauber HNeumann ABaleeiro FCFRaab JfalseMixotrophic chain elongation with syngas and lactate as electron donors.Feeding microbial communities with both organic and inorganic substrates can improve sustainability and feasibility of chain elongation processes. Sustainably produced H₂ , CO₂ , and CO can be co-fed to microorganisms as a source for acetyl-CoA, while a small amount of an ATP-generating organic substrate helps overcome the kinetic hindrances associated with autotrophic carboxylate production. Here, we operated two semi-continuous bioreactor systems with continuous recirculation of H₂ , CO₂ , and CO while co-feeding an organic model feedstock (lactate and acetate) to understand how a mixotrophic community is shaped during carboxylate production. Contrary to the assumption that H₂ , CO₂ , and CO support chain elongation via et2023-01-01T00:00:00Z2023 Feb2026-05-10T06:54:31.908Z2025-04-07T03:15:40.827ZS-EPMC98715303637849110.1111/1751-7915.14163