biostudies-literatureUnknown13Dzofou Ngoumelah DApart from their archetypic use in anaerobic digestion (AD) methanogenic archaea are targeted for a wide range of applications. Using different methanogenic archaea for one specific application requires the optimization of culture media to enable the growth of different strains under identical environmental conditions, e.g., in microbial electrochemical technologies (MET) for (bio)electromethanation. Here we present a new culture medium (BFS01) adapted from the DSM-120 medium by omitting resazurin, yeast extract, casitone, and using a low salt concentration, that was optimized for Methanosarcina barkeri, Methanobacterium formicicum, and Methanothrix soehngenii. The aim was to provide a medium for follow-up co-culture studies using specific methanogens and Geobacter<Frontiers in microbiology1046260https://www.ebi.ac.uk/biostudies/studies/S-EPMC9871610biostudies-literatureA unified and simple medium for growing model methanogens.PMC9871610Wentzel ADzofou Ngoumelah DHarnisch FHeggeset TMBKretzschmar JAune IHSulheim SfalseA unified and simple medium for growing model methanogens.Apart from their archetypic use in anaerobic digestion (AD) methanogenic archaea are targeted for a wide range of applications. Using different methanogenic archaea for one specific application requires the optimization of culture media to enable the growth of different strains under identical environmental conditions, e.g., in microbial electrochemical technologies (MET) for (bio)electromethanation. Here we present a new culture medium (BFS01) adapted from the DSM-120 medium by omitting resazurin, yeast extract, casitone, and using a low salt concentration, that was optimized for Methanosarcina barkeri, Methanobacterium formicicum, and Methanothrix soehngenii. The aim was to provide a medium for follow-up co-culture studies using specific methanogens and Geobacter<2022-01-01T00:00:00Z20222026-05-27T10:51:06.734Z2024-12-03T18:09:49.383ZS-EPMC98716103670456610.3389/fmicb.2022.1046260