biostudies-literatureUnknown13Nguyen H<h4>Introduction</h4>Previous studies verify the formation of enzymatically post-translationally modified (PTM) self-peptides and their preferred recognition by T cells in subjects with type 1 diabetes (T1D). However, questions remain about the relative prevalence of T cells that recognize PTM self-peptides derived from different antigens, their functional phenotypes, and whether their presence correlates with a specific disease endotype.<h4>Methods</h4>To address this question, we identified a cohort of subjects with T1D who had diverse levels of residual beta cell function. Using previously developed HLA class II tetramer reagents, we enumerated T cells that recognize PTM GAD epitopes in the context of DRB1*04:01 or PTM IA2 epitopes in the context of DQB1*03:02 (DQ8).<h4>Results</h4>ConsFrontiers in immunology1015855https://www.ebi.ac.uk/biostudies/studies/S-EPMC9871889biostudies-literatureCharacterizing T cell responses to enzymatically modified beta cell neo-epitopes.PMC9871889Arribas-Layton DChow ITNguyen HKwok WWSpeake CHessner MJGreenbaum CJJames EAfalseCharacterizing T cell responses to enzymatically modified beta cell neo-epitopes.<h4>Introduction</h4>Previous studies verify the formation of enzymatically post-translationally modified (PTM) self-peptides and their preferred recognition by T cells in subjects with type 1 diabetes (T1D). However, questions remain about the relative prevalence of T cells that recognize PTM self-peptides derived from different antigens, their functional phenotypes, and whether their presence correlates with a specific disease endotype.<h4>Methods</h4>To address this question, we identified a cohort of subjects with T1D who had diverse levels of residual beta cell function. Using previously developed HLA class II tetramer reagents, we enumerated T cells that recognize PTM GAD epitopes in the context of DRB1*04:01 or PTM IA2 epitopes in the context of DQB1*03:02 (DQ8).<h4>Results</h4>Cons2022-01-01T00:00:00Z20222025-04-22T06:20:44.649Z2025-04-05T21:40:19.857ZS-EPMC98718893670397510.3389/fimmu.2022.1015855