biostudies-literatureUnknown22(1)Fleischer JRHerzzentrum Göttingen<h4>Background</h4>Colorectal cancer liver metastases (CRCLM) are associated with a poor prognosis, reflected by a five-year survival rate of 14%. Anti-angiogenic therapy through anti-VEGF antibody administration is one of the limited therapies available. However, only a subgroup of metastases uses sprouting angiogenesis to secure their nutrients and oxygen supply, while others rely on vessel co-option (VCO). The distinct mode of vascularization is reflected by specific histopathological growth patterns (HGPs), which have proven prognostic and predictive significance. Nevertheless, their molecular mechanisms are poorly understood.<h4>Methods</h4>We evaluated CRCLM from 225 patients regarding their HGP and clinical data. Moreover, we performed spatial (21,804 spots) and single-cell (22,419 Molecular cancer17https://www.ebi.ac.uk/biostudies/studies/S-EPMC9872436biostudies-literatureMolecular differences of angiogenic versus vessel co-opting colorectal cancer liver metastases at single-cell resolution.PMC9872436Conradi LCDe Oliveira TKuffer SPlanque MFleischer JRFendt SMKarras PJBeißbarth TVermeulen PTeuwen LAKalucka JZeisberg EMBohnenberger HHaas GBleckmann ASchmitt AMXu XGhadimi MfalseMolecular differences of angiogenic versus vessel co-opting colorectal cancer liver metastases at single-cell resolution.<h4>Background</h4>Colorectal cancer liver metastases (CRCLM) are associated with a poor prognosis, reflected by a five-year survival rate of 14%. Anti-angiogenic therapy through anti-VEGF antibody administration is one of the limited therapies available. However, only a subgroup of metastases uses sprouting angiogenesis to secure their nutrients and oxygen supply, while others rely on vessel co-option (VCO). The distinct mode of vascularization is reflected by specific histopathological growth patterns (HGPs), which have proven prognostic and predictive significance. Nevertheless, their molecular mechanisms are poorly understood.<h4>Methods</h4>We evaluated CRCLM from 225 patients regarding their HGP and clinical data. Moreover, we performed spatial (21,804 spots) and single-cell (22,419 2023-01-01T00:00:00Z2023 Jan2025-04-25T22:34:53.094Z2025-04-06T09:08:10.678ZS-EPMC98724363669102810.1186/s12943-023-01713-1