biostudies-literatureArasimowicz-Jelonek MNarodowe Centrum NaukiNarodowe Centrum Nauki (National Science Centre)36-44https://www.ebi.ac.uk/biostudies/studies/S-EPMC9873566biostudies-literatureUnknown9(1)Nitroxyl (HNO) is the one-electron reduced and protonated congener of nitric oxide (•NO), owning a distinct chemical profile. Based on real-time detection, we demonstrate that HNO is endogenously formed in Arabidopsis. Senescence and hypoxia induce shifts in the redox balance, triggering HNO decay or formation mediated by non-enzymatic •NO/HNO interconversion with cellular reductants. The stimuli-dependent HNO generation supports or competes with •NO signalling, depending on the local redox environment.Nature plantsDiscovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana.PMC9873566UMO-2017/26/E/NZ4/00226Bruce King SRebis TGajewska JMilczarek GJagodzik PArasimowicz-Jelonek MZywicki MDoctorovich FFloryszak-Wieczorek JSobieszczuk-Nowicka ESuarez SfalseDiscovery of endogenous nitroxyl as a new redox player in Arabidopsis thaliana.Nitroxyl (HNO) is the one-electron reduced and protonated congener of nitric oxide (•NO), owning a distinct chemical profile. Based on real-time detection, we demonstrate that HNO is endogenously formed in Arabidopsis. Senescence and hypoxia induce shifts in the redox balance, triggering HNO decay or formation mediated by non-enzymatic •NO/HNO interconversion with cellular reductants. The stimuli-dependent HNO generation supports or competes with •NO signalling, depending on the local redox environment.2023-01-01T00:00:00Z2023 Jan2026-05-10T06:54:34.948Z2025-04-07T03:15:11.847ZS-EPMC98735663656463210.1038/s41477-022-01301-z