{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(4)"],"submitter":["Zhang K"],"pubmed_abstract":["<b><i>Background</i>:</b> The currently available methods for sexing human or mouse cells have weaknesses. Therefore, it is necessary to establish new methods. <b><i>Methods</i>:</b> We used bioinformatics approach to identify genes that have alleles on both the X and Y chromosomes of mouse and human genomes and have a region showing a significant difference between the X and Y alleles. We then used polymerase chain reactions (PCR) followed by visualization of the PCR amplicons in agarose gels to establish these genomic regions as genetic sex markers. <b><i>Results</i>:</b> Our bioinformatics analyses identified eight mouse sex markers and 56 human sex markers that are new, <i>i.e</i>. are previously unreported. Six of the eight mouse markers and 14 of the 56 human markers were verified us"],"journal":["Current genomics"],"pagination":["275-288"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9875541"],"repository":["biostudies-literature"],"pubmed_title":["Establishment of New Genetic Markers and Methods for Sex Determination of Mouse and Human Cells using Polymerase Chain Reactions and Crude DNA Samples."],"pmcid":["PMC9875541"],"pubmed_authors":["Cheng S","Ran Q","Yang J","Zhang K","Lou D","Qin Z","Huang H","Ma H","Lu T","Zellmer L","Liao DJ"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of New Genetic Markers and Methods for Sex Determination of Mouse and Human Cells using Polymerase Chain Reactions and Crude DNA Samples.","description":"<b><i>Background</i>:</b> The currently available methods for sexing human or mouse cells have weaknesses. Therefore, it is necessary to establish new methods. <b><i>Methods</i>:</b> We used bioinformatics approach to identify genes that have alleles on both the X and Y chromosomes of mouse and human genomes and have a region showing a significant difference between the X and Y alleles. We then used polymerase chain reactions (PCR) followed by visualization of the PCR amplicons in agarose gels to establish these genomic regions as genetic sex markers. <b><i>Results</i>:</b> Our bioinformatics analyses identified eight mouse sex markers and 56 human sex markers that are new, <i>i.e</i>. are previously unreported. Six of the eight mouse markers and 14 of the 56 human markers were verified us","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-25T19:21:47.196Z","creation":"2025-04-06T07:56:47.692Z"},"accession":"S-EPMC9875541","cross_references":{"pubmed":["36777874"],"doi":["10.2174/1389202923666220610121344"]}}