biostudies-literatureUnknown23(4)Zhang K<b><i>Background</i>:</b> The currently available methods for sexing human or mouse cells have weaknesses. Therefore, it is necessary to establish new methods. <b><i>Methods</i>:</b> We used bioinformatics approach to identify genes that have alleles on both the X and Y chromosomes of mouse and human genomes and have a region showing a significant difference between the X and Y alleles. We then used polymerase chain reactions (PCR) followed by visualization of the PCR amplicons in agarose gels to establish these genomic regions as genetic sex markers. <b><i>Results</i>:</b> Our bioinformatics analyses identified eight mouse sex markers and 56 human sex markers that are new, <i>i.e</i>. are previously unreported. Six of the eight mouse markers and 14 of the 56 human markers were verified usCurrent genomics275-288https://www.ebi.ac.uk/biostudies/studies/S-EPMC9875541biostudies-literatureEstablishment of New Genetic Markers and Methods for Sex Determination of Mouse and Human Cells using Polymerase Chain Reactions and Crude DNA Samples.PMC9875541Cheng SRan QYang JZhang KLou DQin ZHuang HMa HLu TZellmer LLiao DJfalseEstablishment of New Genetic Markers and Methods for Sex Determination of Mouse and Human Cells using Polymerase Chain Reactions and Crude DNA Samples.<b><i>Background</i>:</b> The currently available methods for sexing human or mouse cells have weaknesses. Therefore, it is necessary to establish new methods. <b><i>Methods</i>:</b> We used bioinformatics approach to identify genes that have alleles on both the X and Y chromosomes of mouse and human genomes and have a region showing a significant difference between the X and Y alleles. We then used polymerase chain reactions (PCR) followed by visualization of the PCR amplicons in agarose gels to establish these genomic regions as genetic sex markers. <b><i>Results</i>:</b> Our bioinformatics analyses identified eight mouse sex markers and 56 human sex markers that are new, <i>i.e</i>. are previously unreported. Six of the eight mouse markers and 14 of the 56 human markers were verified us2022-01-01T00:00:00Z2022 Aug2025-04-25T19:21:47.196Z2025-04-06T07:56:47.692ZS-EPMC98755413677787410.2174/1389202923666220610121344