biostudies-literatureLiu LYAHA Grant-in-AidNIAID NIH HHSNHLBI NIH HHSNIH805-12https://www.ebi.ac.uk/biostudies/studies/S-EPMC9875669biostudies-literatureUnknown70(7)<h4>Background</h4>Priming with cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) enhances eosinophil migration and exacerbates the excessive accumulation of eosinophils within the bronchial mucosa of asthmatics. However, mechanisms that drive GM-CSF priming are incompletely understood. Notch signaling is an evolutionarily conserved pathway that regulates cellular processes, including migration, by integrating exogenous and cell-intrinsic cues. This study investigates the hypothesis that the priming-induced enhanced migration of human eosinophils requires the Notch signaling pathway.<h4>Methods</h4>Using pan Notch inhibitors and newly developed human antibodies that specifically neutralize Notch receptor 1 activation, we investigated a role for Notch signaling in AllergyNotch signaling mediates granulocyte-macrophage colony-stimulating factor priming-induced transendothelial migration of human eosinophils.PMC9875669R01 HL095699R37 AI020241Xenakis JJLiu LYWang HSpencer LAfalseNotch signaling mediates granulocyte-macrophage colony-stimulating factor priming-induced transendothelial migration of human eosinophils.<h4>Background</h4>Priming with cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF) enhances eosinophil migration and exacerbates the excessive accumulation of eosinophils within the bronchial mucosa of asthmatics. However, mechanisms that drive GM-CSF priming are incompletely understood. Notch signaling is an evolutionarily conserved pathway that regulates cellular processes, including migration, by integrating exogenous and cell-intrinsic cues. This study investigates the hypothesis that the priming-induced enhanced migration of human eosinophils requires the Notch signaling pathway.<h4>Methods</h4>Using pan Notch inhibitors and newly developed human antibodies that specifically neutralize Notch receptor 1 activation, we investigated a role for Notch signaling in 2015-01-01T00:00:00Z2015 Jul2025-04-04T00:02:57.226Z2025-04-04T00:02:57.226ZS-EPMC98756692584633910.1111/all.12624