{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang J"],"funding":["Coalition for Epidemic Preparedness Innovations (CEPI)","Coalition for Epidemic Preparedness Innovations"],"pagination":["1455-1464"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9878482"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["415(8)"],"pubmed_abstract":["COVID-19 is caused by SARS-CoV-2 infection and remains one of the biggest pandemics around the world since 2019. Vaccination has proved to be an effective way of preventing SARS-CoV-2 infection and alleviating the hospitalization burden. Among different forms of COVID-19 vaccine design, the spike protein of SARS-CoV-2 virus is widely used as a candidate vaccine antigen. As a surface protein on the virus envelop, the spike was reported to be heavily N-glycosylated and glycosylation had a great impact on its immunogenicity and efficacy. Besides, N-glycosylation might vary greatly on different expression systems and sequence variant designs. Therefore, comprehensive analysis of spike N-glycosylation is of great significance for better vaccine understanding and quality control. In this study, "],"journal":["Analytical and bioanalytical chemistry"],"pubmed_title":["In-depth characterization of protein N-glycosylation for a COVID-19 variant-design vaccine spike protein."],"pmcid":["PMC9878482"],"funding_grant_id":["RRZE2101"],"pubmed_authors":["Huang J","Zhou C","An J","Hou S"],"additional_accession":[]},"is_claimable":false,"name":"In-depth characterization of protein N-glycosylation for a COVID-19 variant-design vaccine spike protein.","description":"COVID-19 is caused by SARS-CoV-2 infection and remains one of the biggest pandemics around the world since 2019. Vaccination has proved to be an effective way of preventing SARS-CoV-2 infection and alleviating the hospitalization burden. Among different forms of COVID-19 vaccine design, the spike protein of SARS-CoV-2 virus is widely used as a candidate vaccine antigen. As a surface protein on the virus envelop, the spike was reported to be heavily N-glycosylated and glycosylation had a great impact on its immunogenicity and efficacy. Besides, N-glycosylation might vary greatly on different expression systems and sequence variant designs. Therefore, comprehensive analysis of spike N-glycosylation is of great significance for better vaccine understanding and quality control. In this study, ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Mar","modification":"2026-05-28T03:48:11.626Z","creation":"2024-12-03T21:40:31.079Z"},"accession":"S-EPMC9878482","cross_references":{"pubmed":["36698045"],"doi":["10.1007/s00216-023-04533-w"]}}