{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zeng L"],"funding":["NIDCR NIH HHS","HHS | NIH | National Institute of Dental and Craniofacial Research"],"pagination":["e0035222"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9879115"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["205(1)"],"pubmed_abstract":["Spontaneous mutants with defects in the primary glucose phosphotransferase permease (<i>manLMNO</i>) of Streptococcus sanguinis SK36 showed enhanced fitness at low pH. Transcriptomics and metabolomics with a <i>manL</i> deletion mutant (SK36/<i>manL</i>) revealed redirection of pyruvate to production of acetate and formate, rather than lactate. These observations were consistent with measurements of decreased lactic acid accumulation and increased excretion of acetate, formate, pyruvate, and H<sub>2</sub>O<sub>2</sub>. Genes showing increased expression in SK36/<i>manL</i> included those encoding carbohydrate transporters, extracellular glycosidases, intracellular polysaccharide metabolism, and arginine deiminase and pathways for metabolism of acetoin, ethanolamine, ascorbate, and formate,"],"journal":["Journal of bacteriology"],"pubmed_title":["Glucose Phosphotransferase System Modulates Pyruvate Metabolism, Bacterial Fitness, and Microbial Ecology in Oral Streptococci."],"pmcid":["PMC9879115"],"funding_grant_id":["DE012236","R01 DE012236"],"pubmed_authors":["Taylor ZA","Burne RA","Walker AR","Zeng L"],"additional_accession":[]},"is_claimable":false,"name":"Glucose Phosphotransferase System Modulates Pyruvate Metabolism, Bacterial Fitness, and Microbial Ecology in Oral Streptococci.","description":"Spontaneous mutants with defects in the primary glucose phosphotransferase permease (<i>manLMNO</i>) of Streptococcus sanguinis SK36 showed enhanced fitness at low pH. Transcriptomics and metabolomics with a <i>manL</i> deletion mutant (SK36/<i>manL</i>) revealed redirection of pyruvate to production of acetate and formate, rather than lactate. These observations were consistent with measurements of decreased lactic acid accumulation and increased excretion of acetate, formate, pyruvate, and H<sub>2</sub>O<sub>2</sub>. Genes showing increased expression in SK36/<i>manL</i> included those encoding carbohydrate transporters, extracellular glycosidases, intracellular polysaccharide metabolism, and arginine deiminase and pathways for metabolism of acetoin, ethanolamine, ascorbate, and formate,","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-06-13T06:14:34.624Z","creation":"2025-02-19T04:20:54.237Z"},"accession":"S-EPMC9879115","cross_references":{"pubmed":["36468868"],"doi":["10.1128/jb.00352-22"]}}