{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tapia-Sidas DA"],"funding":["CINVESTAV","Consejo Nacional de Ciencia y Tecnología, Mexico","Agencia Mexicana de Cooperación para el Desarrollo (AMEXCID), Secretaría de Relaciones Exteriores Mexico"],"pagination":["e0279681"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9879405"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(1)"],"pubmed_abstract":["The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide. Public health strategies to reduce viral transmission are based on widespread diagnostic testing to detect and isolate contagious patients. Several reverse transcription (RT)-PCR tests, along with other SARS-CoV-2 diagnostic assays, are available to attempt to cover the global demand. Loop-mediated isothermal amplification (LAMP) based methods have been established as rapid, accurate, point of care diagnostic tests for viral infections; hence, they represent an excellent alternative for SARS-CoV-2 detection. The aim of this study was to develop and describe molecular detection systems for SARS-CoV-2 based on RT-LAMP. Recombinant DNA polymerase from Bacillus ste"],"journal":["PloS one"],"pubmed_title":["Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP."],"pmcid":["PMC9879405"],"funding_grant_id":["483659","2020-1, AMEXCID Mexico-Uruguay","754736"],"pubmed_authors":["Vargas-Hernandez BY","Tapia-Sidas DA","Brieba LG","Lopez-Macias C","Xoconostle-Cazares B","Ruiz-Medrano R","Lira-Carmona R","Nunez-Munoz LA","Calderon-Perez B","Ferat-Osorio E","Gonzalez-Gonzalez R","Ramirez-Pool JA"],"additional_accession":[]},"is_claimable":false,"name":"Starting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP.","description":"The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide. Public health strategies to reduce viral transmission are based on widespread diagnostic testing to detect and isolate contagious patients. Several reverse transcription (RT)-PCR tests, along with other SARS-CoV-2 diagnostic assays, are available to attempt to cover the global demand. Loop-mediated isothermal amplification (LAMP) based methods have been established as rapid, accurate, point of care diagnostic tests for viral infections; hence, they represent an excellent alternative for SARS-CoV-2 detection. The aim of this study was to develop and describe molecular detection systems for SARS-CoV-2 based on RT-LAMP. Recombinant DNA polymerase from Bacillus ste","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-07-14T21:10:44.272Z","creation":"2025-04-06T08:17:59.874Z"},"accession":"S-EPMC9879405","cross_references":{"pubmed":["36701313"],"doi":["10.1371/journal.pone.0279681"]}}