biostudies-literatureTapia-Sidas DACINVESTAVConsejo Nacional de Ciencia y Tecnología, MexicoAgencia Mexicana de Cooperación para el Desarrollo (AMEXCID), Secretaría de Relaciones Exteriores Mexicoe0279681https://www.ebi.ac.uk/biostudies/studies/S-EPMC9879405biostudies-literatureUnknown18(1)The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide. Public health strategies to reduce viral transmission are based on widespread diagnostic testing to detect and isolate contagious patients. Several reverse transcription (RT)-PCR tests, along with other SARS-CoV-2 diagnostic assays, are available to attempt to cover the global demand. Loop-mediated isothermal amplification (LAMP) based methods have been established as rapid, accurate, point of care diagnostic tests for viral infections; hence, they represent an excellent alternative for SARS-CoV-2 detection. The aim of this study was to develop and describe molecular detection systems for SARS-CoV-2 based on RT-LAMP. Recombinant DNA polymerase from Bacillus stePloS oneStarting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP.PMC98794054836592020-1, AMEXCID Mexico-Uruguay754736Vargas-Hernandez BYTapia-Sidas DABrieba LGLopez-Macias CXoconostle-Cazares BRuiz-Medrano RLira-Carmona RNunez-Munoz LACalderon-Perez BFerat-Osorio EGonzalez-Gonzalez RRamirez-Pool JAfalseStarting from scratch: Step-by-step development of diagnostic tests for SARS-CoV-2 detection by RT-LAMP.The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people worldwide. Public health strategies to reduce viral transmission are based on widespread diagnostic testing to detect and isolate contagious patients. Several reverse transcription (RT)-PCR tests, along with other SARS-CoV-2 diagnostic assays, are available to attempt to cover the global demand. Loop-mediated isothermal amplification (LAMP) based methods have been established as rapid, accurate, point of care diagnostic tests for viral infections; hence, they represent an excellent alternative for SARS-CoV-2 detection. The aim of this study was to develop and describe molecular detection systems for SARS-CoV-2 based on RT-LAMP. Recombinant DNA polymerase from Bacillus ste2023-01-01T00:00:00Z20232026-07-14T21:10:44.272Z2025-04-06T08:17:59.874ZS-EPMC98794053670131310.1371/journal.pone.0279681