{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Faust TE"],"funding":["NIA NIH HHS","NIMH NIH HHS","NINDS NIH HHS"],"pubmed_abstract":["Cre/LoxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. The field of microglial biology has particularly benefited from this technology as microglia have historically been difficult to transduce with virus or electroporation methods for gene delivery. Here, we interrogate four of the most widely available microglial inducible Cre lines. We demonstrate varying degrees of recombination efficiency and spontaneous recombination, depending on the Cre line and loxP distance. We also establish best practice guidelines and protocols to measure recombination efficiency in microglia, which could be extended to other cell types. There is increasing evidence that microglia are key regulators of neural circuit struct"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.01.09.523268"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9881995"],"repository":["biostudies-literature"],"pubmed_title":["A comparative analysis of microglial inducible Cre lines."],"pmcid":["PMC9881995"],"funding_grant_id":["R01 MH118329","R01 NS106721","RF1 AG068558","R01 MH113743","RF1 AG068281","R01 AG072489","R01 NS117533"],"pubmed_authors":["Knobeloch KP","Feinberg PA","Strasburger H","Masuda T","Prinz M","Chan A","Faust TE","O'Connor C","Kawaguchi R","Schaefer A","Schafer DP","Amann L"],"additional_accession":[]},"is_claimable":false,"name":"A comparative analysis of microglial inducible Cre lines.","description":"Cre/LoxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. The field of microglial biology has particularly benefited from this technology as microglia have historically been difficult to transduce with virus or electroporation methods for gene delivery. Here, we interrogate four of the most widely available microglial inducible Cre lines. We demonstrate varying degrees of recombination efficiency and spontaneous recombination, depending on the Cre line and loxP distance. We also establish best practice guidelines and protocols to measure recombination efficiency in microglia, which could be extended to other cell types. There is increasing evidence that microglia are key regulators of neural circuit struct","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-19T17:17:31.738Z","creation":"2025-04-19T17:17:31.738Z"},"accession":"S-EPMC9881995","cross_references":{"pubmed":["36711492"],"doi":["10.1101/2023.01.09.523268"]}}