biostudies-literatureUnknownMarchand TNIDDK NIH HHSNHLBI NIH HHSNCI NIH HHSNIGMS NIH HHSSkeletal stem cells have been isolated from various tissues, including periosteum and bone marrow, where they exhibit key functions in bone biology and hematopoiesis, respectively. The role of periosteal skeletal stem cells in bone regeneration and healing has been extensively studied, but their ability to contribute to the bone marrow stroma is still under debate. In the present study, we characterized a whole bone transplantation model that mimics the initial bone marrow necrosis and fatty infiltration seen after injury. Using this model and a lineage tracing approach, we observed the migration of periosteal skeletal stem cells into the bone marrow after transplantation. Once in the bone marrow, periosteal skeletal stem cells are phenotypically and functionally reprogrammed into bone marbioRxiv : the preprint server for biology2023.01.12.523842https://www.ebi.ac.uk/biostudies/studies/S-EPMC9882153biostudies-literaturePeriosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injury.PMC9882153R01 DK130895P30 CA013330R01 HL162584R01 DK112976T32 GM007288R56 DK130895R01 DK056638Pinho SSaint-Vanne JMaryanovich MFrenette PSAkinnola KETakeishi SGritsman KBirbrair AMarchand TLamy TTarte KfalsePeriosteal skeletal stem cells can migrate into the bone marrow and support hematopoiesis after injury.Skeletal stem cells have been isolated from various tissues, including periosteum and bone marrow, where they exhibit key functions in bone biology and hematopoiesis, respectively. The role of periosteal skeletal stem cells in bone regeneration and healing has been extensively studied, but their ability to contribute to the bone marrow stroma is still under debate. In the present study, we characterized a whole bone transplantation model that mimics the initial bone marrow necrosis and fatty infiltration seen after injury. Using this model and a lineage tracing approach, we observed the migration of periosteal skeletal stem cells into the bone marrow after transplantation. Once in the bone marrow, periosteal skeletal stem cells are phenotypically and functionally reprogrammed into bone mar2025-01-01T00:00:00Z2025 Jan2026-04-08T05:44:16.955Z2026-04-07T22:39:51.779ZS-EPMC98821533671192710.1101/2023.01.12.523842